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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0015252,
umls-concept:C0028630,
umls-concept:C0028778,
umls-concept:C0040077,
umls-concept:C0086045,
umls-concept:C0146894,
umls-concept:C0150312,
umls-concept:C0205463,
umls-concept:C0243071,
umls-concept:C0332206,
umls-concept:C0443199,
umls-concept:C0728940,
umls-concept:C1524075,
umls-concept:C1955880
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pubmed:issue |
12
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pubmed:dateCreated |
2000-11-30
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pubmed:abstractText |
Removal of 2',3'-didehydro-3'-deoxythymidine-5'-monophosphate (d4TMP) from a blocked DNA chain can occur through transfer of the chain-terminating residue to a nucleotide acceptor by human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). ATP-dependent removal of either d4TMP or 3'-azido-3'-deoxythymidine-5'-monophosphate (AZTMP) is increased in AZT resistant HIV-1 RT (containing D67N/K70R/T215F/K219Q mutations). Removal of d4TMP is strongly inhibited by the next complementary deoxynucleoside triphosphate (50% inhibitory concentration [IC(50)] of approximately 0.5 microM), whereas removal of AZTMP is much less sensitive to this inhibition (IC(50) of >100 microM). This could explain the lack of cross-resistance by AZT-resistant HIV-1 to d4T in phenotypic drug susceptibility assays.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10222017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10438382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10445025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10509572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10534734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10602755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10669364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10708277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10722492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-10839665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-115082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-1706277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-2334156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-2460925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-2479733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-342082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-55415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-6975780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7214019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7509634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7522429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7682554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7692440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7693703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-7963708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-8141575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-8175673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-8517697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-8585744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9108961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9153415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9456262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9527771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9758571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9811824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9831551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11083661-9843396
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',3'-dideoxy-2',3'-didehydrothymidi...,
http://linkedlifedata.com/resource/pubmed/chemical/3'-azido-3'-deoxythymidine...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dideoxynucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Reverse Transcriptase,
http://linkedlifedata.com/resource/pubmed/chemical/Reverse Transcriptase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Stavudine,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Thymine Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Zidovudine
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3465-72
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pubmed:dateRevised |
2010-9-29
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pubmed:meshHeading |
pubmed-meshheading:11083661-Adenosine Triphosphate,
pubmed-meshheading:11083661-DNA Primers,
pubmed-meshheading:11083661-Dideoxynucleotides,
pubmed-meshheading:11083661-HIV Reverse Transcriptase,
pubmed-meshheading:11083661-Reverse Transcriptase Inhibitors,
pubmed-meshheading:11083661-Stavudine,
pubmed-meshheading:11083661-Templates, Genetic,
pubmed-meshheading:11083661-Thymidine Monophosphate,
pubmed-meshheading:11083661-Thymine Nucleotides,
pubmed-meshheading:11083661-Zidovudine
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pubmed:year |
2000
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pubmed:articleTitle |
Differential removal of thymidine nucleotide analogues from blocked DNA chains by human immunodeficiency virus reverse transcriptase in the presence of physiological concentrations of 2'-deoxynucleoside triphosphates.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Miami, Miami, Florida 33101-6129, USA.
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