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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
2001-1-2
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pubmed:abstractText |
The activity of the [-831; +103] promoter of the human cyclooxygenase-2 gene in cultured rabbit chondrocytes is stimulated 2.9 +/- 0.3-fold by interleukin-1beta and this stimulation depends on [-132; -124] C/EBP binding-and [-223; -214] NF-kappaB binding-sites. The C/EBPbeta and C/EBPdelta factors bind to the [-132; -124] sequence. The [-61; -53] sequence is also recognized by C/EBPbeta and C/EBPdelta as well as USF. Mutation of the whole [-61; -53] sequence abolished the stimulation of transcription but single mutations of the C/EBP or USF site did not alter the activity of the promoter, suggesting that the factors bound to the proximal [-61; -53] sequence interact with different members of the general transcription machinery. The [-223; -214] site binds only the p50/p50 homodimer and a non-rel-related protein, but not the transcriptionally active heterodimer p50/p65. The p50/p50 homodimer could interact with the C/EBP family members bound to the [-132; -124] sequence for full stimulation of the COX-2 transcription by interleukin-1beta in chondrocytes. By contrast, the [-448; -449] sequence binds with a low affinity both the p50/p50 homodimeric and p50/p65 heterodimeric forms of NF-kappaB but has no role in the regulation of the human COX-2 promoter in chondrocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-beta,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-delta,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CEBPD protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-rel,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6798-809
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11082190-Animals,
pubmed-meshheading:11082190-Base Sequence,
pubmed-meshheading:11082190-Binding Sites,
pubmed-meshheading:11082190-CCAAT-Enhancer-Binding Protein-beta,
pubmed-meshheading:11082190-CCAAT-Enhancer-Binding Protein-delta,
pubmed-meshheading:11082190-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:11082190-Cartilage, Articular,
pubmed-meshheading:11082190-Cell Nucleus,
pubmed-meshheading:11082190-Cells, Cultured,
pubmed-meshheading:11082190-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:11082190-Chondrocytes,
pubmed-meshheading:11082190-Cyclooxygenase 2,
pubmed-meshheading:11082190-Female,
pubmed-meshheading:11082190-Gene Deletion,
pubmed-meshheading:11082190-Gene Expression Regulation,
pubmed-meshheading:11082190-Humans,
pubmed-meshheading:11082190-Interleukin-1,
pubmed-meshheading:11082190-Isoenzymes,
pubmed-meshheading:11082190-Kinetics,
pubmed-meshheading:11082190-Membrane Proteins,
pubmed-meshheading:11082190-Models, Biological,
pubmed-meshheading:11082190-Molecular Sequence Data,
pubmed-meshheading:11082190-Mutagenesis,
pubmed-meshheading:11082190-Plasmids,
pubmed-meshheading:11082190-Promoter Regions, Genetic,
pubmed-meshheading:11082190-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:11082190-Protein Binding,
pubmed-meshheading:11082190-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:11082190-Rabbits,
pubmed-meshheading:11082190-Sequence Homology, Nucleic Acid,
pubmed-meshheading:11082190-Transcription, Genetic,
pubmed-meshheading:11082190-Transcription Factors
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pubmed:year |
2000
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pubmed:articleTitle |
Critical role of C/EBPdelta and C/EBPbeta factors in the stimulation of the cyclooxygenase-2 gene transcription by interleukin-1beta in articular chondrocytes.
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pubmed:affiliation |
UPRES-A CNRS 7079, Université Pierre et Marie Curie, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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