Source:http://linkedlifedata.com/resource/pubmed/id/11078873
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2000-12-5
|
| pubmed:abstractText |
Bovine kidney lysosomal alpha-mannosidase is a family 38 alpha-mannosidase involved in the degradation of glycoproteins. The mechanism-based reagent, 5-fluoro-beta-L-gulosyl fluoride, was used to trap a glycosyl-enzyme intermediate, thereby labelling the catalytic nucleophile of this enzyme. After proteolytic digestion and high performance liquid chromatography/tandem mass spectrometry (MS) analysis, a labelled peptide was localised, and the sequence: HIDPFGHSRE determined by fragmentation tandem MS analysis. Taking into consideration sequence alignments of this region with those of other alpha-mannosidases of the same family, this result strongly suggests that the catalytic nucleophile in this enzyme is Asp197.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0014-5793
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
484
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
175-8
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11078873-Amino Acid Sequence,
pubmed-meshheading:11078873-Animals,
pubmed-meshheading:11078873-Aspartic Acid,
pubmed-meshheading:11078873-Binding Sites,
pubmed-meshheading:11078873-Catalytic Domain,
pubmed-meshheading:11078873-Cattle,
pubmed-meshheading:11078873-Chromatography, High Pressure Liquid,
pubmed-meshheading:11078873-Humans,
pubmed-meshheading:11078873-Kidney,
pubmed-meshheading:11078873-Kinetics,
pubmed-meshheading:11078873-Lysosomes,
pubmed-meshheading:11078873-Mannosidases,
pubmed-meshheading:11078873-Mass Spectrometry,
pubmed-meshheading:11078873-Molecular Sequence Data,
pubmed-meshheading:11078873-Rats,
pubmed-meshheading:11078873-Sequence Alignment,
pubmed-meshheading:11078873-Sequence Homology, Amino Acid,
pubmed-meshheading:11078873-Swine,
pubmed-meshheading:11078873-alpha-Mannosidase
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Identification of Asp197 as the catalytic nucleophile in the family 38 alpha-mannosidase from bovine kidney lysosomes.
|
| pubmed:affiliation |
Department of Chemistry, University of British Columbia, Vancouver, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|