| pubmed-article:11078653 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C0027571 | lld:lifeskim |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C0441587 | lld:lifeskim |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:11078653 | lifeskim:mentions | umls-concept:C0684192 | lld:lifeskim |
| pubmed-article:11078653 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11078653 | pubmed:dateCreated | 2000-12-13 | lld:pubmed |
| pubmed-article:11078653 | pubmed:abstractText | A useful method for inserting any DNA fragment into the chromosome of Neisseriae has been developed. The method relies on recombination-proficient vector plasmid pNLE1, a pUC19 derivative containing (1) genes conferring resistance to ampicillin and erythromycin, as selectable markers; (2) a chromosomal region necessary for its integration into the Neisseria chromosome; (3) a specific uptake sequence which is required for natural transformation; (4) a promoter capable of functioning in Neisseria; and (5) several unique restriction sites useful for cloning. pNLE1 integrates into the leuS region of the neisserial chromosome at high frequencies by transformation-mediated recombination. The usefulness of this vector has been demonstrated by cloning the tetracycline-resistance gene (tet) and subsequently inserting the tet gene into the meningococcal chromosome. | lld:pubmed |
| pubmed-article:11078653 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11078653 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11078653 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11078653 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11078653 | pubmed:issn | 0147-619X | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:BruniC BCB | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:SalvatorePP | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:AlifanoPP | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:BucciCC | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:LavitolaAA | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:PagliaruloCC | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:TrediciMM | lld:pubmed |
| pubmed-article:11078653 | pubmed:author | pubmed-author:CantalupoGG | lld:pubmed |
| pubmed-article:11078653 | pubmed:copyrightInfo | Copyright 2000 Academic Press. | lld:pubmed |
| pubmed-article:11078653 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11078653 | pubmed:volume | 44 | lld:pubmed |
| pubmed-article:11078653 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11078653 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11078653 | pubmed:pagination | 275-9 | lld:pubmed |
| pubmed-article:11078653 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
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| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
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| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
| pubmed-article:11078653 | pubmed:meshHeading | pubmed-meshheading:11078653... | lld:pubmed |
| pubmed-article:11078653 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11078653 | pubmed:articleTitle | A new vector for insertion of any DNA fragment into the chromosome of transformable Neisseriae. | lld:pubmed |
| pubmed-article:11078653 | pubmed:affiliation | Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano,", Università di Napoli "Federico II,", Centro di Endocrinologia ed Oncologia Sperimentale "G. Salvatore" of the Consiglio Nazionale delle Ricerche, Naples, Italy. | lld:pubmed |
| pubmed-article:11078653 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11078653 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11078653 | lld:pubmed |
