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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-12-13
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pubmed:abstractText |
A useful method for inserting any DNA fragment into the chromosome of Neisseriae has been developed. The method relies on recombination-proficient vector plasmid pNLE1, a pUC19 derivative containing (1) genes conferring resistance to ampicillin and erythromycin, as selectable markers; (2) a chromosomal region necessary for its integration into the Neisseria chromosome; (3) a specific uptake sequence which is required for natural transformation; (4) a promoter capable of functioning in Neisseria; and (5) several unique restriction sites useful for cloning. pNLE1 integrates into the leuS region of the neisserial chromosome at high frequencies by transformation-mediated recombination. The usefulness of this vector has been demonstrated by cloning the tetracycline-resistance gene (tet) and subsequently inserting the tet gene into the meningococcal chromosome.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0147-619X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11078653-Ampicillin,
pubmed-meshheading:11078653-Chromosome Mapping,
pubmed-meshheading:11078653-Chromosomes, Bacterial,
pubmed-meshheading:11078653-DNA, Bacterial,
pubmed-meshheading:11078653-DNA Transposable Elements,
pubmed-meshheading:11078653-Erythromycin,
pubmed-meshheading:11078653-Genetic Vectors,
pubmed-meshheading:11078653-Neisseria meningitidis,
pubmed-meshheading:11078653-Penicillins,
pubmed-meshheading:11078653-Promoter Regions, Genetic,
pubmed-meshheading:11078653-Protein Synthesis Inhibitors,
pubmed-meshheading:11078653-Recombination, Genetic,
pubmed-meshheading:11078653-Tetracycline,
pubmed-meshheading:11078653-Transformation, Genetic
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pubmed:year |
2000
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pubmed:articleTitle |
A new vector for insertion of any DNA fragment into the chromosome of transformable Neisseriae.
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pubmed:affiliation |
Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano,", Università di Napoli "Federico II,", Centro di Endocrinologia ed Oncologia Sperimentale "G. Salvatore" of the Consiglio Nazionale delle Ricerche, Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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