Linked Life Data

11076996

Source:http://linkedlifedata.com/resource/pubmed/id/11076996

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-1-2
pubmed:abstractText
Cultures of human blood lymphocytes from three subjects were incubated with the clastogen mitomycin C (MMC, 500 ng/ml) and the aneugen diethylstilboestrol (DES, 80 microM) 23 h before harvesting, to induce formation of micronuclei (MN) and numerical and structural alterations in metaphase chromosomes. We used fluorescence in situ hybridization (FISH) with painting probes for all human chromosomes to determine which chromosomes had contributed material to the induced MN. MMC treatment induced an approximately 18-fold increase in MN and led to a significant increase in hypodiploidy and structural chromosome aberrations in metaphase preparations. Undercondensation of pericentromeric heterochromatin of chromosomes 9 and 1 occurred in 20-75% of metaphases and FISH disclosed an abundance of material from these chromosomes in induced MN (62-69% from chromosome 9 and 7-12% from chromosome 1). DES treatment of lymphocytes induced a seven-fold increase in MN frequency and four-fold increase in the frequency of numerical aberrations; structural aberrations were not significantly increased. FISH analysis showed that material from all chromosomes was present in DES-induced MN, with material from chromosome 1 present in 16% of MN and material from each other chromosomes being present in 2-10% of MN. Material from chromosomes 14, 19 and 21 was significantly more frequent material from chromosome Y significantly less frequent in DES-treated cells than in controls. The findings of the MMC studies indicate that the heterochromatin block of chromosome 9 is a specific target for MMC-induced undercondensation, which induces a preferential occurrence of chromosome 9 material in MN. DES, in contrast, does not trigger heterochromatin decondensation and fails to induce such a significant appearance of material of particular chromosomes in MN.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0267-8357
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
459-67
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed-meshheading:11076996-Adult, pubmed-meshheading:11076996-Antibiotics, Antineoplastic, pubmed-meshheading:11076996-Antineoplastic Agents, Hormonal, pubmed-meshheading:11076996-Carcinogens, pubmed-meshheading:11076996-Cells, Cultured, pubmed-meshheading:11076996-Chromosome Aberrations, pubmed-meshheading:11076996-Chromosome Painting, pubmed-meshheading:11076996-Chromosomes, Human, Pair 1, pubmed-meshheading:11076996-Chromosomes, Human, Pair 9, pubmed-meshheading:11076996-Diethylstilbestrol, pubmed-meshheading:11076996-Female, pubmed-meshheading:11076996-Heterochromatin, pubmed-meshheading:11076996-Humans, pubmed-meshheading:11076996-In Situ Hybridization, Fluorescence, pubmed-meshheading:11076996-Indoles, pubmed-meshheading:11076996-Lymphocytes, pubmed-meshheading:11076996-Male, pubmed-meshheading:11076996-Micronuclei, Chromosome-Defective, pubmed-meshheading:11076996-Mitomycin, pubmed-meshheading:11076996-Mutation, pubmed-meshheading:11076996-Plasmids, pubmed-meshheading:11076996-Ploidies
pubmed:year
2000
pubmed:articleTitle
Chromosome painting reveals specific patterns of chromosome occurrence in mitomycin C- and diethylstilboestrol-induced micronuclei.
pubmed:affiliation
Abt. Humanbiologie und Humangenetik der Universität, Postfach 3049, D-67653 Kaiserslautern, Germany. efauth@rhrk.uni-kl.de
pubmed:publicationType
Journal Article