Linked Life Data

11075960

Source:http://linkedlifedata.com/resource/pubmed/id/11075960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-2-16
pubmed:abstractText
Ceramide has emerged as a pleiotropic signal mediator of cellular responses including differentiation, proliferation, cell cycle arrest and apoptosis. In the present study we evaluated the effect of cell permeant ceramide analogues on ligand-induced tyrosine phosphorylation of the EGF receptor (EGFR), phospholipase Cy (PLCgamma) activity and cell proliferation. Treatment with N-acetylsphingosine (C2-cer) and N-hexanoylceramide (C6-cer) prevented EGF-induced tyrosine trans-phosphorylation of the receptor in two different cell lines overexpressing the human EGFR (A431 and EGF-T17 cells). In contrast, treatment of A431 and EGFR-T17 cells with C2-cer or C6-cer did not affect the ligand binding capacity of the receptor, an effect that was however observed after TPA-induced activation of PKC. In addition EGF-stimulated PLCgamma activity was transiently decreased in A431 cells treated with C6-cer and only a modest, albeit significant reduction on ligand-induced 3H-InsP3 generation was observed in EGFR-T17 cells pretreated with ceramide. We also examined the effect of C2-cer on serum (A431)- or EGF (EGFR-T 17)-induced cell proliferation. Treatment of EGFR-TI7 cells with C2-cer (0.1-10 microM) did not affect cell viability, but prevented EGF-induced 3H-thymidine incorporation in a dose-dependent manner. In contrast, 3H-thymidine incorporation in serum-stimulated A431 cells decreased only at the higher doses of C2-cer used (1-10 microM), being this effect accompanied by a slight, albeit significant (20-25%), reduction in cell viability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0145-5680
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1305-12
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11075960-Animals, pubmed-meshheading:11075960-Cell Division, pubmed-meshheading:11075960-Cell Line, pubmed-meshheading:11075960-Cell Membrane Permeability, pubmed-meshheading:11075960-Cell Survival, pubmed-meshheading:11075960-Ceramides, pubmed-meshheading:11075960-Enzyme Activation, pubmed-meshheading:11075960-Epidermal Growth Factor, pubmed-meshheading:11075960-Humans, pubmed-meshheading:11075960-Inositol Phosphates, pubmed-meshheading:11075960-Isoenzymes, pubmed-meshheading:11075960-Mice, pubmed-meshheading:11075960-Mitogens, pubmed-meshheading:11075960-Phospholipase C gamma, pubmed-meshheading:11075960-Phosphorylation, pubmed-meshheading:11075960-Phosphotyrosine, pubmed-meshheading:11075960-Protein Binding, pubmed-meshheading:11075960-Receptor, Epidermal Growth Factor, pubmed-meshheading:11075960-Signal Transduction, pubmed-meshheading:11075960-Tetradecanoylphorbol Acetate, pubmed-meshheading:11075960-Type C Phospholipases
pubmed:year
2000
pubmed:articleTitle
Regulation by ceramide of epidermal growth factor signal transduction and mitogenesis in cell lines overexpressing the growth factor receptor.
pubmed:affiliation
Departamento de Bioquímica, Biología Molecular y Fisiología, Universidad de Las Palmas de Gran Canaria, Spain. gallardo@cicei.ulpgc.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't