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rdf:type |
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lifeskim:mentions |
umls-concept:C0018270,
umls-concept:C0020792,
umls-concept:C0031715,
umls-concept:C0037083,
umls-concept:C0080298,
umls-concept:C0205145,
umls-concept:C0379710,
umls-concept:C0450240,
umls-concept:C1333708,
umls-concept:C1515655,
umls-concept:C1706210,
umls-concept:C1709305,
umls-concept:C1710082,
umls-concept:C2347567
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pubmed:issue |
11
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pubmed:dateCreated |
2001-3-12
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pubmed:databankReference |
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pubmed:abstractText |
Caveolin-1 was first identified as a phosphoprotein in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. Tyrosine 14 is now thought to be the principal site for recognition by c-Src kinase; however, little is known about this phosphorylation event. Here, we generated a monoclonal antibody (mAb) probe that recognizes only tyrosine 14-phosphorylated caveolin-1. Using this approach, we show that caveolin-1 (Y14) is a specific tyrosine kinase substrate that is constitutively phosphorylated in Src- and Abl-transformed cells and transiently phosphorylated in a regulated fashion during growth factor signaling. We also provide evidence that tyrosine-phosphorylated caveolin-1 is localized at the major sites of tyrosine-kinase signaling, i.e. focal adhesions. By analogy with other signaling events, we hypothesized that caveolin-1 could serve as a docking site for pTyr-binding molecules. In support of this hypothesis, we show that phosphorylation of caveolin-1 on tyrosine 14 confers binding to Grb7 (an SH2-domain containing protein) both in vitro and in vivo. Furthermore, we demonstrate that binding of Grb7 to tyrosine 14-phosphorylated caveolin-1 functionally augments anchorage-independent growth and epidermal growth factor (EGF)-stimulated cell migration. We discuss the possible implications of our findings in the context of signal transduction.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/CAV1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cav1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Caveolins,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GRB7 Adaptor Protein,
http://linkedlifedata.com/resource/pubmed/chemical/GRB7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Grb7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates,
http://linkedlifedata.com/resource/pubmed/chemical/src-Family Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0888-8809
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pubmed:author |
pubmed-author:BouzahzahBB,
pubmed-author:BregmanD BDB,
pubmed-author:Campos-GonzalezRR,
pubmed-author:GalbiatiFF,
pubmed-author:IyengarPP,
pubmed-author:LeeHH,
pubmed-author:LisantiM PMP,
pubmed-author:LublinD MDM,
pubmed-author:PestellR GRG,
pubmed-author:SchererP EPE,
pubmed-author:VolonteDD,
pubmed-author:WilsonM TMT
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1750-75
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11075810-3T3 Cells,
pubmed-meshheading:11075810-Adipocytes,
pubmed-meshheading:11075810-Amino Acid Sequence,
pubmed-meshheading:11075810-Animals,
pubmed-meshheading:11075810-Antibodies, Monoclonal,
pubmed-meshheading:11075810-Caveolae,
pubmed-meshheading:11075810-Caveolin 1,
pubmed-meshheading:11075810-Caveolins,
pubmed-meshheading:11075810-Cell Adhesion,
pubmed-meshheading:11075810-Cell Division,
pubmed-meshheading:11075810-Cell Movement,
pubmed-meshheading:11075810-Epidermal Growth Factor,
pubmed-meshheading:11075810-Female,
pubmed-meshheading:11075810-GRB7 Adaptor Protein,
pubmed-meshheading:11075810-Growth Substances,
pubmed-meshheading:11075810-Humans,
pubmed-meshheading:11075810-Insulin,
pubmed-meshheading:11075810-Lipid Metabolism,
pubmed-meshheading:11075810-Mice,
pubmed-meshheading:11075810-Mice, Inbred BALB C,
pubmed-meshheading:11075810-Molecular Sequence Data,
pubmed-meshheading:11075810-Phosphorylation,
pubmed-meshheading:11075810-Proteins,
pubmed-meshheading:11075810-Receptor, Epidermal Growth Factor,
pubmed-meshheading:11075810-Signal Transduction,
pubmed-meshheading:11075810-Tyrosine,
pubmed-meshheading:11075810-Vanadates,
pubmed-meshheading:11075810-src-Family Kinases
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pubmed:year |
2000
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pubmed:articleTitle |
Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: identification of a c-Src/Cav-1/Grb7 signaling cassette.
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pubmed:affiliation |
Department of Molecular Pharmacology and The Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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