Linked Life Data

11074255

Source:http://linkedlifedata.com/resource/pubmed/id/11074255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-11-27
pubmed:databankReference
pubmed:abstractText
Human C1qR(P) is a highly glycosylated transmembrane protein that is the human C1q receptor/receptor component that in vitro mediates enhancement of Fc- and C3b-mediated phagocytosis. A human genomic clone and a murine genomic clone that is 73% identical in sequence with the coding region for human C1qR(P) cDNA have been isolated. Chromosomal localization of the human and murine gene demonstrates that these genes are syntenic. Murine cell lines of diverse myeloid origins are shown to respond to interaction of C1q with the enhancement of phagocytosis similar to that seen previously in human peripheral blood monocytes. Northern blot, RT-PCR, Western blot and FACS analyses demonstrated that mC1qR(P) is expressed in these murine myeloid cell lines, but not in a mouse epithelial cell line, similar to the cell type expression of the human gene product. A polyclonal antibody to a peptide sequence common to the deduced sequence from the both murine and human C1qR(P) inhibited the enhancement of phagocytosis response to C1q when cells were permeabilized to permit access of the antibody to the intracellular milieu. These data support the postulate that the identified murine and human genes are homologs, confirm the previously predicted intracellular location of the C-terminus of the molecule, and indicates the necessary role of this intracellular domain in transducing the signal that leads to enhancement of phagocytic function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
377-89
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11074255-Amino Acid Sequence, pubmed-meshheading:11074255-Animals, pubmed-meshheading:11074255-Antigens, CD44, pubmed-meshheading:11074255-Base Sequence, pubmed-meshheading:11074255-Carrier Proteins, pubmed-meshheading:11074255-Cell Line, pubmed-meshheading:11074255-Chromosome Mapping, pubmed-meshheading:11074255-Complement C1q, pubmed-meshheading:11074255-DNA, Complementary, pubmed-meshheading:11074255-Flow Cytometry, pubmed-meshheading:11074255-Gene Expression Profiling, pubmed-meshheading:11074255-Humans, pubmed-meshheading:11074255-Membrane Glycoproteins, pubmed-meshheading:11074255-Mice, pubmed-meshheading:11074255-Microscopy, Fluorescence, pubmed-meshheading:11074255-Mitochondrial Proteins, pubmed-meshheading:11074255-Molecular Sequence Data, pubmed-meshheading:11074255-Phagocytosis, pubmed-meshheading:11074255-RNA, Messenger, pubmed-meshheading:11074255-Rabbits, pubmed-meshheading:11074255-Receptors, Complement, pubmed-meshheading:11074255-Sheep, pubmed-meshheading:11074255-U937 Cells
pubmed:year
2000
pubmed:articleTitle
Characterization of the murine homolog of C1qR(P): identical cellular expression pattern, chromosomal location and functional activity of the human and murine C1qR(P).
pubmed:affiliation
Department of Molecular Biology and Biochemistry, 3205 Biological Sciences II, University of California, Irvine, CA 92697-3900, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't