Source:http://linkedlifedata.com/resource/pubmed/id/11072249
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2000-11-14
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| pubmed:abstractText |
Presentation of cell-associated antigen to T cells is a critical event in the initiation of an anti-tumor immune response but it appears to often be deficient or limiting. Here we report an experimental system for stimulation of human T lymphocytes using autologous antigen presenting cells (APCs) and autologous tumor cells. Two types of APCs were prepared from human bone marrow: MC and DC. MC were produced by using GM-CSF and SCF. DC were obtained with the same cytokines plus IL-4. DC and MC were generated in parallel from the same patients and their phenotypes and capacities to prime T lymphocytes were analyzed and compared. MC were CD14+, CD1a-, CD33+ and HLA-DR+. Two populations of DC were defined: immature DC were uniformly CD1a-; mature DC expressed CD1a, CD80, CD86, HLA-DR, CD54 and CD58 but lacked surface CD14. Stimulation of autologous T lymphocytes was studied by measuring their proliferation and cytotoxic function. In more than 80% of our experiments the proliferation of autologous T lymphocytes cocultured with APC pulsed or not with tumor cell lysates was higher than that of T cells cultured alone. DC were more effective than MC in stimulating proliferation of lymphocytes. The capacity of a patient's autologous bone marrow-derived APC to stimulate T cells when exposed to autologous tumor cell lysates suggest that such antigen-exposed APC may be useful in specific anti-tumor immunotherapy protocols.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
0020-7136
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
88
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
783-90
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| pubmed:dateRevised |
2007-7-24
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| pubmed:meshHeading |
pubmed-meshheading:11072249-Antigen-Presenting Cells,
pubmed-meshheading:11072249-Antigens, Neoplasm,
pubmed-meshheading:11072249-Antigens, Surface,
pubmed-meshheading:11072249-Autoantigens,
pubmed-meshheading:11072249-Bone Marrow,
pubmed-meshheading:11072249-Coculture Techniques,
pubmed-meshheading:11072249-Cytotoxicity, Immunologic,
pubmed-meshheading:11072249-Dendritic Cells,
pubmed-meshheading:11072249-Humans,
pubmed-meshheading:11072249-Immunophenotyping,
pubmed-meshheading:11072249-Kidney Neoplasms,
pubmed-meshheading:11072249-Lymphocyte Activation,
pubmed-meshheading:11072249-Macrophages,
pubmed-meshheading:11072249-Melanoma,
pubmed-meshheading:11072249-T-Lymphocyte Subsets,
pubmed-meshheading:11072249-T-Lymphocytes,
pubmed-meshheading:11072249-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:11072249-Tumor Cells, Cultured,
pubmed-meshheading:11072249-Tumor Necrosis Factor-alpha
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
In vitro immunization of patient T cells with autologous bone marrow antigen presenting cells pulsed with tumor lysates.
|
| pubmed:affiliation |
Laboratory of Immunology, Institut Bergonié, Bordeaux, France. coulon@bergonie.org
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|