Source:http://linkedlifedata.com/resource/pubmed/id/11071658
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2000-11-27
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pubmed:abstractText |
Selectin-dependent rolling is the earliest observable event in the recruitment of leukocytes to inflamed tissues. Several glycoproteins decorated with sialic acid, fucose, and/or sulfate have been shown to bind the selectins. The best-characterized selectin ligand is P-selectin glycoprotein-1 (PSGL-1) that supports P-selectin- dependent rolling in vitro and in vivo. In vitro studies have suggested that PSGL-1 may also be a ligand for E- and L-selectins. To study the in vivo function of PSGL-1, without the influence of other leukocyte proteins, the authors observed the interaction of PSGL-1-coated microspheres in mouse venules stimulated to express P- and/or E-selectin. Microspheres coated with functional recombinant PSGL-1 rolled in surgically stimulated and tumor necrosis factor alpha (TNFalpha)-stimulated mouse venules. P-selectin deficiency or inhibition abolished microsphere rolling in surgically and TNFalpha-stimulated venules, whereas E-selectin deficiency or inhibition increased microsphere rolling velocity in TNFalpha-stimulated venules. The results suggest that P-selectin-PSGL-1 interaction alone is sufficient to mediate rolling in vivo and that E-selectin-PSGL-1 interaction supports slow rolling.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/P-selectin ligand protein,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3585-91
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11071658-Animals,
pubmed-meshheading:11071658-E-Selectin,
pubmed-meshheading:11071658-Hemodynamics,
pubmed-meshheading:11071658-Humans,
pubmed-meshheading:11071658-Leukocytes,
pubmed-meshheading:11071658-Male,
pubmed-meshheading:11071658-Membrane Glycoproteins,
pubmed-meshheading:11071658-Mice,
pubmed-meshheading:11071658-Mice, Inbred C57BL,
pubmed-meshheading:11071658-Mice, Knockout,
pubmed-meshheading:11071658-Microspheres,
pubmed-meshheading:11071658-Models, Animal,
pubmed-meshheading:11071658-Neutrophils,
pubmed-meshheading:11071658-P-Selectin,
pubmed-meshheading:11071658-Time Factors,
pubmed-meshheading:11071658-Tumor Necrosis Factor-alpha,
pubmed-meshheading:11071658-Venules
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pubmed:year |
2000
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pubmed:articleTitle |
P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo.
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pubmed:affiliation |
Cardiovascular Research Group and Section of Surgery, Division of Clinical Sciences (NGH), University of Sheffield, United Kingdom. k.norman@sheffield.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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