Linked Life Data

11070352

Source:http://linkedlifedata.com/resource/pubmed/id/11070352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-12-5
pubmed:abstractText
Here are described the effects of androgens, and other molecules known to bind to androgen receptors (AR), on MOP cells established from the human prostate cancer cell line LNCaP. MOP cells contained AR: 100000 binding sites/cell, K(D) for 5alpha dihydrotestosterone (DHT) 0.5 nM, size 110 kDa. The AR gene has the same repetition polymorphism in exon 1 and the T876A mutation in exon 8 as LNCaP. The proliferation of MOP cells in culture was repressed by the synthetic androgen 17beta-hydroxy-17-methyl-estra-4,9,11-trien-3-one (R 1881), the antiandrogen cyproterone acetate (CYPA), estradiol (E2), progesterone and the synthetic progestin promegestone: 17,21 dimethyl-19 nor-4,9 pregnandiene-3,20 dione (R 5020). The number of cells recovered after 7 days decreased to approximately 40% of controls. ED(70)s ranged between 50 pM for R 1881 to 50 nM for E2 and CYPA. Treatment with R 1881 decreased [3H]thymidine incorporation into DNA and increased dramatically the doubling time. R 1881, CYPA and E2 blocked the cell cycle between G1 and S phases and they induced apototosis as demonstrated by the increase of blebs on the plasma membrane, nuclear fragmentation, TdT-mediated dUTP nick end-labeling (TUNEL)-positive cells and internucleosomal DNA breaks. In athymic nude mice, testosterone enanthate prevented the growth of MOP tumors and, when tumors did develop, brought about regression. However, the tumors did not regress completely and finally escaped treatment. In conclusion, a variant of the LNCaP cell line has been established. With these cells it was possible to confirm that androgens paradoxically repress the growth of some prostate cancer cells both in culture and in vivo. In addition it is demonstrated in culture but not in vivo, for the first time to the authors' knowledge, that a synthetic androgen is able to induce apoptosis of cells established from human prostate carcinoma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0960-0760
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-49
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11070352-Androgens, pubmed-meshheading:11070352-Animals, pubmed-meshheading:11070352-Apoptosis, pubmed-meshheading:11070352-Cell Cycle, pubmed-meshheading:11070352-Cell Division, pubmed-meshheading:11070352-Cyproterone Acetate, pubmed-meshheading:11070352-Estradiol, pubmed-meshheading:11070352-Exons, pubmed-meshheading:11070352-Humans, pubmed-meshheading:11070352-Male, pubmed-meshheading:11070352-Metribolone, pubmed-meshheading:11070352-Mice, pubmed-meshheading:11070352-Mice, Nude, pubmed-meshheading:11070352-Point Mutation, pubmed-meshheading:11070352-Polymorphism, Genetic, pubmed-meshheading:11070352-Promegestone, pubmed-meshheading:11070352-Prostatic Neoplasms, pubmed-meshheading:11070352-Receptors, Androgen, pubmed-meshheading:11070352-Testosterone Congeners, pubmed-meshheading:11070352-Tumor Cells, Cultured, pubmed-meshheading:11070352-Xenograft Model Antitumor Assays
pubmed:articleTitle
Inhibition of growth and induction of apoptosis by androgens of a variant of LNCaP cell line.
pubmed:affiliation
INSERM-U 329 Hôpital Debrousse, 29 Rue Soeur Bouvier, 69322 Cédex 05, Lyon, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't