Linked Life Data

11069933

Source:http://linkedlifedata.com/resource/pubmed/id/11069933

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2000-11-14
pubmed:abstractText
To understand the physiological role of kainate receptors and their participation in seizure induction in animal models of epilepsy, it will be necessary to develop a comprehensive description of their action in the CA3 region of the hippocampus. Activation of presynaptic kainate receptors depresses excitatory synaptic transmission at mossy fiber and associational-commissural inputs to CA3 pyramidal neurons (Vignes et al., 1998; Bortolotto et al., 1999; Kamiya and Ozawa, 2000). In this study, we use gene-targeted mice lacking glutamate receptor 5 (GluR5) or GluR6 kainate receptor subunits to identify the receptor subunits that comprise the kainate receptors responsible for presynaptic modulation of CA3 transmission. We found that bath application of kainate (3 microm) profoundly reduced EPSCs at mossy fiber and collateral synapses in neurons from wild-type and GluR5(-/-) mice but had no effect on EPSCs in neurons from GluR6(-/-) mice. These results therefore contrast with previous studies that supported a role for GluR5-containing receptors at mossy fiber and associational-commissural synapses (Vignes et al., 1998; Bortolotto et al., 1999). Surprisingly, at perforant path synapses kainate receptor activation enhanced transmission; this potentiation was abolished in both GluR5 and GluR6 knock-out mice. Kainate receptors thus play multiple and complex roles to modulate excitatory synaptic transmission in the CA3 region of the hippocampus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8269-78
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11069933-Animals, pubmed-meshheading:11069933-Excitatory Amino Acid Agonists, pubmed-meshheading:11069933-Excitatory Amino Acid Antagonists, pubmed-meshheading:11069933-Excitatory Postsynaptic Potentials, pubmed-meshheading:11069933-GABA Antagonists, pubmed-meshheading:11069933-GABA-A Receptor Antagonists, pubmed-meshheading:11069933-Hippocampus, pubmed-meshheading:11069933-Kainic Acid, pubmed-meshheading:11069933-Mice, pubmed-meshheading:11069933-Mice, Inbred Strains, pubmed-meshheading:11069933-Mice, Knockout, pubmed-meshheading:11069933-Mossy Fibers, Hippocampal, pubmed-meshheading:11069933-Neural Inhibition, pubmed-meshheading:11069933-Neurons, pubmed-meshheading:11069933-Patch-Clamp Techniques, pubmed-meshheading:11069933-Perforant Pathway, pubmed-meshheading:11069933-Receptors, Kainic Acid, pubmed-meshheading:11069933-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:11069933-Receptors, Presynaptic, pubmed-meshheading:11069933-Synapses, pubmed-meshheading:11069933-Synaptic Transmission
pubmed:year
2000
pubmed:articleTitle
Identification of the kainate receptor subunits underlying modulation of excitatory synaptic transmission in the CA3 region of the hippocampus.
pubmed:affiliation
Molecular Neurobiology Laboratory and Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA. contractor@salk.edu
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't