Linked Life Data

11068340

Source:http://linkedlifedata.com/resource/pubmed/id/11068340

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-12-28
pubmed:abstractText
We have prepared a monoclonal antibody, denoted as C-4, which specifically recognizes astroglia with radial forms in the adult zebrafish brain. This report suggests that there are at least two different types of astroglia in the mature teleost brain, only one of which is recognized by C-4. Further, we have found that the C-4-binding astroglia are comprised of three morphologically distinct cellular types. Immunoblot analysis revealed that the antibody recognized only one protein band of approximately 30 kDa in the membrane fraction of the adult zebrafish brain. In the spinal cord, stained glial cells appeared to occur in the same location as ependymocytes. The processes and cell bodies of extra-ependymal cells, many adjacent to ependymocytes and a few near the pial surface, were also stained by the antibody. In the cerebellum, long processes stained by C-4 were found in the molecular layer, and these connected the cerebellum surface to the Purkinje-like cell layer. Long processes were also stained in the mesencephalon, but these were thicker than those in the spinal cord and they linked the two ventricles. The optic tectum, olfactory bulb and cranial nerves, including the optic nerves, were, however, completely devoid of the C-4 antigen. Double-immunofluorescence with antibodies against glial fibrillary acidic protein (GFAP) and C-4 demonstrated that C-4-positive cells were also GFAP-positive, although there was also a subset of GFAP-positive cells which were C-4-negative. The C-4 antibody is thus a useful tool for studying subtypes of GFAP-positive astroglia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-4864
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-28
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
A monoclonal antibody stains radial glia in the adult zebrafish (Danio rerio) CNS.
pubmed:affiliation
Department of Biology, Faculty of Science, Okayama University, Okayama 700-8530, Japan.
pubmed:publicationType
Journal Article