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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1979-9-17
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pubmed:abstractText |
The major outer membrane proteins from 10 gonococcal strains were examined after 125I-labeling of the proteins as single bands resolved by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. These 125I-proteins were then treated with either trypsin or alpha-chymotrypsin, and the resultant 125I-peptides were visualized by autoradiography after two-dimensional electrophoretic and chromatographic separation on thin-layer cellulose sheets. Several 125I-peptides were present in all the major outer membrane proteins examined. The presence and absence of additional 125I-peptides segregated the major proteins into two pattern groups. One group consisted of major outer membranes with molecular weights of 34,000 or 33,000; major proteins with molecular weights of 32,000 constituted the other group. Two beta-lactamase-producing gonococcal isolates were examined. Their major outer membrane proteins were identical in apparent molecular weights and alpha-chymotryptic 125I-peptide fingerprints; these proteins contained 125I-peptides not found in other gonococcal major proteins. No 125I-peptide differences were found among the major outer membrane proteins of strain F62 gonococci that exhibited differences in piliation and/or colony opacity characteristics.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-101459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-211086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-4134571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-4152046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-416164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-5516455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-5640817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-56416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-78895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/110681-893422
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
799-810
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Studies on gonococcus infection. XVIII. 125I-labeled peptide mapping of the major protein of the gonococcal cell wall outer membrane.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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