|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0030705,
umls-concept:C0032520,
umls-concept:C0205210,
umls-concept:C0348026,
umls-concept:C0368790,
umls-concept:C0370003,
umls-concept:C0752235,
umls-concept:C1511790,
umls-concept:C1522609,
umls-concept:C1554112,
umls-concept:C2936894
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|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2000-11-8
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|
pubmed:abstractText |
The aim of the study is to evaluate the diagnostic sensitivity of a 16S ribosomal RNA-based PCR on clinical specimens from patients with erythema migrans (EM) and neuroborreliosis and to compare the sensitivities with those obtained by in vitro culture and serological testing. A semiquantitative detection system, representing the input amount of specific DNA and thus the density of spirochetes in clinical specimens, indicated the preferred clinical sample to obtain for PCR testing.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
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pubmed:issn |
1084-8592
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-50
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11066015-Adolescent,
pubmed-meshheading:11066015-Adult,
pubmed-meshheading:11066015-Aged,
pubmed-meshheading:11066015-Aged, 80 and over,
pubmed-meshheading:11066015-Borrelia burgdorferi Group,
pubmed-meshheading:11066015-Colony Count, Microbial,
pubmed-meshheading:11066015-DNA, Bacterial,
pubmed-meshheading:11066015-Erythema Chronicum Migrans,
pubmed-meshheading:11066015-Female,
pubmed-meshheading:11066015-Genes, rRNA,
pubmed-meshheading:11066015-Humans,
pubmed-meshheading:11066015-Immunoglobulin G,
pubmed-meshheading:11066015-Immunoglobulin M,
pubmed-meshheading:11066015-Lyme Neuroborreliosis,
pubmed-meshheading:11066015-Male,
pubmed-meshheading:11066015-Middle Aged,
pubmed-meshheading:11066015-Polymerase Chain Reaction,
pubmed-meshheading:11066015-RNA, Ribosomal, 16S,
pubmed-meshheading:11066015-Sensitivity and Specificity,
pubmed-meshheading:11066015-Skin,
pubmed-meshheading:11066015-Spirochaetales
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|
pubmed:year |
2000
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pubmed:articleTitle |
Diagnostic value of PCR for detection of Borrelia burgdorferi DNA in clinical specimens from patients with erythema migrans and Lyme neuroborreliosis.
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|
pubmed:affiliation |
Borrelia Laboratory, Department of Clinical Biochemistry, Statens Seruminstitut, Copenhagen. lebech@dadlnet.dk
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|
