Linked Life Data

11064939

Source:http://linkedlifedata.com/resource/pubmed/id/11064939

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2000-11-9
pubmed:abstractText
A renewable electrochemical immunosensor was developed for the determination of transferrin in human serum. It is based on a paraffin-graphite-transferrin antiserum biocomposite, which needs no additional curing. A competitive binding assay was used to determine transferrin in human serum with the aid of transferrin labeled with horseradish peroxidase. The assay conditions were optimized, including the loading of transferrin antiserum in the biocomposite, the amount of labeled transferrin in the incubation solution, incubation time and temperature. Serum samples were analyzed and the results demonstrate that the concentration range of determination with this system meets the demands of clinical analysis. The surface of the immunosensor can be regenerated by simply polishing to obtain a fresh immunocomposite ready to be used in a new competitive assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-2654
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1595-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Renewable amperometric immunosensor based on paraffin-graphite-transferrin antiserum biocomposite for transferrin assay.
pubmed:affiliation
College of Chemistry and Chemical Engineering, Institute for Chemometrics and Sensing Technology, Hunan University, Changsha, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't