Source:http://linkedlifedata.com/resource/pubmed/id/11064204
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2001-1-5
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pubmed:databankReference | |
pubmed:abstractText |
Plasmids of the H incompatibility complex confer protection against all known channel-forming colicins (PacB character) and resistance to potassium tellurite (Te(r)) to Escherichia coli strains. A DNA clone (2.2 kbp) from plasmid Mip233 (IncHI3) expressing PacB-Te(r) phenotypes was studied. DNA sequence analysis revealed a high degree of homology with the enzyme O-acetylserine sulfhydrylase. Size of the PacB-Te(r) transcript was estimated as 1200 bases. A single polypeptide was found on SDS-polyacrylamide gel with a molecular mass estimated of 34 kDa. The effect of channel-forming colicins and tellurite was analyzed at physiological and transcriptional levels. Results suggest that the pacB gene product could be a reductase-like enzyme. It is also suggested that presence of the PacB character among H plasmid confers selective advantage on cells sharing an ecological niche.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colicins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tellurium
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
192
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
257-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11064204-Amino Acid Sequence,
pubmed-meshheading:11064204-Base Sequence,
pubmed-meshheading:11064204-Binding Sites,
pubmed-meshheading:11064204-Blotting, Northern,
pubmed-meshheading:11064204-Colicins,
pubmed-meshheading:11064204-Drug Resistance, Microbial,
pubmed-meshheading:11064204-Escherichia coli,
pubmed-meshheading:11064204-Molecular Sequence Data,
pubmed-meshheading:11064204-Peptides,
pubmed-meshheading:11064204-Plasmids,
pubmed-meshheading:11064204-RNA, Bacterial,
pubmed-meshheading:11064204-RNA, Messenger,
pubmed-meshheading:11064204-Ribosomes,
pubmed-meshheading:11064204-Tellurium,
pubmed-meshheading:11064204-Transcription, Genetic
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pubmed:year |
2000
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pubmed:articleTitle |
On the mechanism of resistance to channel-forming colicins (PacB) and tellurite, encoded by plasmid Mip233 (IncHI3).
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pubmed:affiliation |
Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, 1041A, Caracas, Venezuela. galonso@reacciun.ve
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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