|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0013735,
umls-concept:C0017337,
umls-concept:C0019397,
umls-concept:C0021585,
umls-concept:C0030943,
umls-concept:C0033684,
umls-concept:C0439148,
umls-concept:C0679058,
umls-concept:C1101096,
umls-concept:C1547699,
umls-concept:C1711351,
umls-concept:C2700640
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2000-11-21
|
|
pubmed:abstractText |
The Drosophila suppressor of position-effect variegation Su(var)3-9 encodes a heterochromatin-associated protein that is evolutionarily conserved. In contrast to its yeast and mammalian orthologs, the Drosophila Su(var)3-9 gene is fused with the locus encoding the gamma subunit of translation initiation factor eIF2. Synthesis of the two unrelated proteins is resolved by alternative splicing. A similar dicistronic Su(var)3-9/eIF-2gamma transcription unit was found in Clytus arietis, Leptinotarsa decemlineata, and Scoliopterix libatrix, representing two different orders of holometabolic insects (Coleoptera and Lepidoptera). In all these species the N terminus of the eIF-2gamma, which is encoded by the first two exons, is fused to SU(VAR)3-9. In contrast to Drosophila melanogaster, RT-PCR analysis in the two coleopteran and the lepidopteran species demonstrated the usage of a nonconserved splice donor site located within the 3' end of the SU(VAR)3-9 ORF, resulting in removal of the Su(var)3-9-specific stop codon from the mRNA and complete in-frame fusion of the SU(VAR)3-9 and eIF-2gamma ORFs. In the centipede Lithobius forficatus eIF-2gamma and Su(var)3-9 are unconnected. Conservation of the dicistronic Su(var)3-9/eIF-2gamma transcription unit in the studied insects indicates its origin before radiation of holometabolic insects and represents a useful tool for molecular phylogenetic analysis in arthropods.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-10511565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-10660052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-1365916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-1633570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-1644277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-3099166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-7556678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-7616258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-7774582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-7915232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-7969472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8057385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8106381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8314581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8413234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8417348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8457211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8743704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8825487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-8947054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9079882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9155028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9254694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9311988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9620780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9628876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9699460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9736774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063691-9847405
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0016-6731
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
156
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1157-67
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11063691-Amino Acid Sequence,
pubmed-meshheading:11063691-Animals,
pubmed-meshheading:11063691-Arthropods,
pubmed-meshheading:11063691-Base Sequence,
pubmed-meshheading:11063691-DNA Primers,
pubmed-meshheading:11063691-Drosophila,
pubmed-meshheading:11063691-Drosophila Proteins,
pubmed-meshheading:11063691-Drosophila melanogaster,
pubmed-meshheading:11063691-Eukaryotic Initiation Factor-2,
pubmed-meshheading:11063691-Evolution, Molecular,
pubmed-meshheading:11063691-Exons,
pubmed-meshheading:11063691-Genes, Insect,
pubmed-meshheading:11063691-Heterochromatin,
pubmed-meshheading:11063691-Insects,
pubmed-meshheading:11063691-Lepidoptera,
pubmed-meshheading:11063691-Molecular Sequence Data,
pubmed-meshheading:11063691-Phylogeny,
pubmed-meshheading:11063691-Protein Subunits,
pubmed-meshheading:11063691-Repressor Proteins,
pubmed-meshheading:11063691-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11063691-Sequence Alignment,
pubmed-meshheading:11063691-Sequence Homology, Amino Acid,
pubmed-meshheading:11063691-Species Specificity
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Two genes become one: the genes encoding heterochromatin protein Su(var)3-9 and translation initiation factor subunit eIF-2gamma are joined to a dicistronic unit in holometabolic insects.
|
|
pubmed:affiliation |
Institute of Genetics, Martin Luther University Halle-Wittenberg, D-06108 Halle, Germany. krauss@rz.uni-leipzig.de
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
