Linked Life Data

11062248

Source:http://linkedlifedata.com/resource/pubmed/id/11062248

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-5-23
pubmed:databankReference
pubmed:abstractText
We have isolated the full-length cDNA of a novel human serine threonine protein kinase gene. The deduced protein sequence contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain containing all the characteristic sequence motifs of serine protein kinases. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. In contrast, it shows only a low degree of sequence similarity to other members of the PKC family. Therefore, the new protein has been termed protein kinase D2 (PKD2). The mRNA of PKD2 is widely expressed in human and murine tissues. It encodes a protein with a molecular mass of 105 kDa in SDS-polyacrylamide gel electrophoresis, which is expressed in various human cell lines, including HL60 cells, which do not express PKCmicro. In vivo phorbol ester binding studies demonstrated a concentration-dependent binding of [(3)H]phorbol 12,13-dibutyrate to PKD2. The addition of phorbol 12,13-dibutyrate in the presence of dioleoylphosphatidylserine stimulated the autophosphorylation of PKD2 in a synergistic fashion. Phorbol esters also stimulated autophosphorylation of PKD2 in intact cells. PKD2 activated by phorbol esters efficiently phosphorylated the exogenous substrate histone H1. In addition, we could identify the C-terminal Ser(876) residue as an in vivo phosphorylation site within PKD2. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. Finally, gastrin was found to be a physiological activator of PKD2 in human AGS-B cells stably transfected with the CCK(B)/gastrin receptor. Thus, PKD2 is a novel phorbol ester- and growth factor-stimulated protein kinase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3310-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11062248-Amino Acid Sequence, pubmed-meshheading:11062248-Carcinogens, pubmed-meshheading:11062248-Cells, Cultured, pubmed-meshheading:11062248-Cloning, Molecular, pubmed-meshheading:11062248-DNA, Complementary, pubmed-meshheading:11062248-Enzyme Activation, pubmed-meshheading:11062248-Growth Substances, pubmed-meshheading:11062248-HL-60 Cells, pubmed-meshheading:11062248-Humans, pubmed-meshheading:11062248-Molecular Sequence Data, pubmed-meshheading:11062248-Molecular Weight, pubmed-meshheading:11062248-Phorbol 12,13-Dibutyrate, pubmed-meshheading:11062248-Phorbol Esters, pubmed-meshheading:11062248-Phosphorylation, pubmed-meshheading:11062248-Protein Kinases, pubmed-meshheading:11062248-Sequence Homology, Amino Acid, pubmed-meshheading:11062248-Serine, pubmed-meshheading:11062248-Signal Transduction, pubmed-meshheading:11062248-Transfection, pubmed-meshheading:11062248-Tritium
pubmed:year
2001
pubmed:articleTitle
Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases.
pubmed:affiliation
Abteilung Innere Medizin I and Institut für Humangenetik, Medizinische Fakultät der Universität, Ulm, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't