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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2000-12-6
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pubmed:abstractText |
The 14-3-3 proteins are associated with proto-oncogene and oncogene products. Here, we generated NIH 3T3 cells overexpressing the beta isoform of the 14-3-3 proteins (14-3-3 beta) to examine the function of this isoform in cellular proliferation and oncogenic transformation. Overexpression of 14-3-3 beta in NIH 3T3 cells stimulated cell growth and supported anchorage-independent growth in soft agar medium and tumor formation in nude mice. To elucidate the molecular mechanisms of 14-3-3 beta-mediated NIH 3T3 transformation, we examined the activity of mitogen-activated protein kinase (MAPK) after serum stimulation. Overexpression of 14-3-3 beta augmented MAPK activity after serum stimulation, and MAPK activity correlated well with the amount of 14-3-3 beta expression. The colony-forming ability of NIH 3T3 cells overexpressing 14-3-3 beta in soft agar medium was efficiently abolished by exogenous expression of a dominant-negative mutant of MEK1 and 14-3-3 beta physically interacted with Raf-1 in these cells. These findings indicate that 14-3-3 beta has oncogenic potential, mainly through enhancement of Raf-1 activation and resultant augmentation of signaling in the MAPK cascade.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0143-3334
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2073-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11062170-14-3-3 Proteins,
pubmed-meshheading:11062170-3T3 Cells,
pubmed-meshheading:11062170-Animals,
pubmed-meshheading:11062170-Cell Division,
pubmed-meshheading:11062170-Cell Transformation, Neoplastic,
pubmed-meshheading:11062170-MAP Kinase Kinase 1,
pubmed-meshheading:11062170-MAP Kinase Signaling System,
pubmed-meshheading:11062170-Mice,
pubmed-meshheading:11062170-Mice, Inbred BALB C,
pubmed-meshheading:11062170-Mice, Nude,
pubmed-meshheading:11062170-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:11062170-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11062170-Phenotype,
pubmed-meshheading:11062170-Protein Isoforms,
pubmed-meshheading:11062170-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11062170-Transfection,
pubmed-meshheading:11062170-Tyrosine 3-Monooxygenase
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pubmed:year |
2000
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pubmed:articleTitle |
Role of the beta isoform of 14-3-3 proteins in cellular proliferation and oncogenic transformation.
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pubmed:affiliation |
Department of Medical Genetics and Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. takihara@biken.osaka-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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