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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2001-1-24
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pubmed:abstractText |
Mammalian degenerin (MDEG) is a member of the amiloride-sensitive sodium ion channel family, and its site-directed active mutant (MDEG-G430F) induces massive Na+ influx into cells, leading to cell ballooning and cell bursting. We attempted a novel therapeutic approach for gastric cancers by transferring MDEG-G430F into cancer cells using tumor-specific promoters. In carcinoembryonic antigen (CEA)-producing gastric cancer cells, the level of cell death observed when MDEG-G430F was used with a CEA promoter was similar to that observed when using a potent nonspecific promoter such as the cytomegalovirus promoter. In an in vivo study, fusogenic liposome complexes containing MDEG-G430F driven by the CEA promoter were injected intraperitoneally into CEA-producing gastric cancer cells in a mouse peritoneal dissemination model. Although all 15 of the control mice were dead by 50 days postinoculation, 13 of the 15 mice treated with MDEG-G430F survived. These results indicate that transferring MDEG-G430F into cancer tissues using tumor-specific promoters can achieve striking and selective cancer cell death irrespective of the transcriptional efficiency of the promoters used in vivo, and suggest that this approach is a promising new strategy for cancer gene therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0929-1903
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pubmed:author |
pubmed-author:EguchiAA,
pubmed-author:HayashiNN,
pubmed-author:HijiokaTT,
pubmed-author:HoriMM,
pubmed-author:HorimotoMM,
pubmed-author:ItoTT,
pubmed-author:IyodaKK,
pubmed-author:MinamiYY,
pubmed-author:NakanishiMM,
pubmed-author:SasakiYY,
pubmed-author:ShimadaSS,
pubmed-author:TohyamaMM,
pubmed-author:ToyamaTT,
pubmed-author:UgawaSS,
pubmed-author:WadaSS,
pubmed-author:YakushijinTT
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1341-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11059692-Animals,
pubmed-meshheading:11059692-Carcinoembryonic Antigen,
pubmed-meshheading:11059692-Cell Survival,
pubmed-meshheading:11059692-Epithelial Sodium Channel,
pubmed-meshheading:11059692-Female,
pubmed-meshheading:11059692-Humans,
pubmed-meshheading:11059692-Injections, Intraperitoneal,
pubmed-meshheading:11059692-Ion Channels,
pubmed-meshheading:11059692-Liposomes,
pubmed-meshheading:11059692-Liver Neoplasms,
pubmed-meshheading:11059692-Luciferases,
pubmed-meshheading:11059692-Mice,
pubmed-meshheading:11059692-Mice, Inbred BALB C,
pubmed-meshheading:11059692-Mice, Nude,
pubmed-meshheading:11059692-Mutagenesis, Site-Directed,
pubmed-meshheading:11059692-Mutation,
pubmed-meshheading:11059692-Neoplasm Transplantation,
pubmed-meshheading:11059692-Nerve Tissue Proteins,
pubmed-meshheading:11059692-Peritoneal Diseases,
pubmed-meshheading:11059692-Stomach Neoplasms,
pubmed-meshheading:11059692-Survival Rate,
pubmed-meshheading:11059692-Time Factors,
pubmed-meshheading:11059692-Transduction, Genetic,
pubmed-meshheading:11059692-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
A novel strategy for cancer therapy by mutated mammalian degenerin gene transfer.
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pubmed:affiliation |
Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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