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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2000-11-3
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pubmed:abstractText |
A new technique of PCR hot start using oligonucleotide primers with a stem-loop structure is developed here. The molecular beacon oligonucleotide structure without any chromophore addition to the ends was used. The 3'-end sequence of the primers was complementary to the target and five or six nucleotides complementary to the 3'-end were added to the 5'-end. During preparation of the reaction mixture and initial heating, the oligonucleotide has a stem-loop structure and cannot serve as an effective primer for DNA polymerase. After heating to the annealing temperature it acquires a linear structure and primer extension can begin.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-10339560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-10495897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-1852616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-2155396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-2946935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-8074881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-8136135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-8424872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-8951376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-9555727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11058144-9630890
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E94
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11058144-Base Pairing,
pubmed-meshheading:11058144-Biosensing Techniques,
pubmed-meshheading:11058144-Coloring Agents,
pubmed-meshheading:11058144-DNA Primers,
pubmed-meshheading:11058144-DNA-Directed DNA Polymerase,
pubmed-meshheading:11058144-Fluorescence,
pubmed-meshheading:11058144-Genes, p53,
pubmed-meshheading:11058144-Hot Temperature,
pubmed-meshheading:11058144-Humans,
pubmed-meshheading:11058144-Lymphocytes,
pubmed-meshheading:11058144-Mycobacterium tuberculosis,
pubmed-meshheading:11058144-Nucleic Acid Conformation,
pubmed-meshheading:11058144-Nucleic Acid Hybridization,
pubmed-meshheading:11058144-Polymerase Chain Reaction,
pubmed-meshheading:11058144-Sensitivity and Specificity
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pubmed:year |
2000
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pubmed:articleTitle |
PCR hot start using primers with the structure of molecular beacons (hairpin-like structure).
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pubmed:affiliation |
St Petersburg Nuclear Physics Institute, Russian Academy of Science, Gatchina 188350, Russia and Tehran Pasteur Institute, Iran. kaboev@omrb.pnpi.spb.ru
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pubmed:publicationType |
Journal Article
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