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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-11-17
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pubmed:abstractText |
MAT1 (ménage à trois1) functions as an assembly/targeting factor of CAK (cyclin-dependent kinase-activating kinase). In a search for MAT1-interacting proteins using yeast two-hybrid system, MCM7 (minichromosome maintenance 7), a member of a family of DNA licensing factors, was identified. The physical interaction between MAT1 and MCM7 was confirmed in vivo in yeast cells and verified with in vitro protein binding assays. Further studies showed the RING-finger motif of MAT1 is not required for the interaction with MCM7, while the C-terminal domain of MAT1 is indispensable. Immunoprecipitation of MCM7 in human osteosarcoma MG63 cells demonstrated that MCM7 associates with the CAK complex in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCNH protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin H,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MCM7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/cyclin-dependent kinase-activating...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
484
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17-21
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11056214-Amino Acid Motifs,
pubmed-meshheading:11056214-Cell Cycle Proteins,
pubmed-meshheading:11056214-Cyclin H,
pubmed-meshheading:11056214-Cyclin-Dependent Kinases,
pubmed-meshheading:11056214-Cyclins,
pubmed-meshheading:11056214-DNA-Binding Proteins,
pubmed-meshheading:11056214-Humans,
pubmed-meshheading:11056214-Macromolecular Substances,
pubmed-meshheading:11056214-Nuclear Proteins,
pubmed-meshheading:11056214-Precipitin Tests,
pubmed-meshheading:11056214-Protein Binding,
pubmed-meshheading:11056214-Protein Structure, Tertiary,
pubmed-meshheading:11056214-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11056214-Substrate Specificity,
pubmed-meshheading:11056214-Tumor Cells, Cultured,
pubmed-meshheading:11056214-Two-Hybrid System Techniques
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pubmed:year |
2000
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pubmed:articleTitle |
The MAT1 cyclin-dependent kinase-activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor.
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pubmed:affiliation |
Department of Molecular Pharmacology and Toxicology, University of Southern California School of Pharmacy, Los Angeles, CA 9--33, USA.
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pubmed:publicationType |
Journal Article
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