Source:http://linkedlifedata.com/resource/pubmed/id/11055868
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2001-2-14
|
| pubmed:abstractText |
The relationship between a retroviral infection and the development of nonviral intracytoplasmic inclusion bodies was studied in a Boa constrictor model. Twelve juvenile age- and size-matched inclusion body disease (IBD)-negative boas were randomly divided into three groups. Each group was inoculated intraperitoneally with 1 ml of an IBD virus (IBDV)-infected liver homogenate or 1 ml of normal boa liver homogenate (sham-inoculated control) or was left untreated. All boas were monitored for development of IBD by daily examination and serial liver biopsy over 1 year. The 4 IBDV-inoculated boas became IBDV and inclusion positive by 10 weeks postinoculation. The average size and density of inclusion bodies increased with the duration of infection. Ultrastructurally, inclusion bodies <2 microm in diameter consisted of intracytoplasmic aggregates of granular electron-dense material that were not membrane limited. Larger inclusions (3-6 microm in diameter) were characterized as membrane-bound aggregates of amorphous to granular electron-dense material admixed with membranelike fragments. The sham-inoculated and untreated control snakes did not become inclusion or IBDV positive. Direct comparison of the protein electrophoretograms of IBDV-infected and normal boa tissues demonstrated a prominent 68-kd protein band unique to infected inclusion-positive tissues. Monoclonal antibodies directed against the 68-kd protein band specifically labeled inclusion bodies. The results of this study demonstrate that IBD inclusions represent an intracytoplasmic accumulation of an antigenically distinct IBDV-associated protein.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0300-9858
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
37
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
449-59
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11055868-Animals,
pubmed-meshheading:11055868-Antigens, Viral,
pubmed-meshheading:11055868-Biopsy,
pubmed-meshheading:11055868-Blotting, Western,
pubmed-meshheading:11055868-Boidae,
pubmed-meshheading:11055868-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11055868-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11055868-Euthanasia,
pubmed-meshheading:11055868-Female,
pubmed-meshheading:11055868-Inclusion Bodies,
pubmed-meshheading:11055868-Kidney,
pubmed-meshheading:11055868-Liver,
pubmed-meshheading:11055868-Mice,
pubmed-meshheading:11055868-Microscopy, Immunoelectron,
pubmed-meshheading:11055868-Molecular Weight,
pubmed-meshheading:11055868-Random Allocation,
pubmed-meshheading:11055868-Retroviridae,
pubmed-meshheading:11055868-Retroviridae Infections
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Isolation and characterization of an antigenically distinct 68-kd protein from nonviral intracytoplasmic inclusions in Boa constrictors chronically infected with the inclusion body disease virus (IBDV: Retroviridae).
|
| pubmed:affiliation |
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|