11055720

Source:http://linkedlifedata.com/resource/pubmed/id/11055720

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0024485, umls-concept:C0150369, umls-concept:C0178862, umls-concept:C0201699, umls-concept:C0392747, umls-concept:C0443172, umls-concept:C0450031
pubmed:issue	20
pubmed:dateCreated	2000-11-7
pubmed:abstractText	Nanoliter-volume proton nuclear magnetic resonance (NMR) spectroscopy is used to monitor the electrolyte temperature during capillary electrophoresis (CE). By measuring the shift in the proton resonance frequency of the water signal, the intracapillary temperature can be recorded noninvasively with subsecond temporal resolution and spatial resolution on the order of 1 mm. Thermal changes of more than 65 degrees C are observed under both equilibrium and nonequilibrium conditions for typical CE separation conditions. Several capillary and buffer combinations are examined with external cooling by both liquid and air convection. Additionally, NMR thermometry allows nonequilibrium temperatures in analyte bands to be monitored during a separation. As one example, a plug of 1 mM NaCl is injected into a capillary filled with 50 mM borate buffer. Upon reaching the NMR detector, the temperature in the NaCl band is more than 20 degrees C higher than the temperature in the surrounding buffer. Such observations have direct applicability to a variety of studies, including experiments which utilize sample stacking and isotachophoresis.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/GM53030
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0370536
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	0003-2700
pubmed:author	pubmed-author:LaceyM EME, pubmed-author:SweedlerJ VJV, pubmed-author:WebbA GAG
pubmed:issnType	Print
pubmed:day	15
pubmed:volume	72
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	4991-8
pubmed:dateRevised	2007-11-14
pubmed:meshHeading	pubmed-meshheading:11055720-Electrophoresis, Capillary, pubmed-meshheading:11055720-Magnetic Resonance Spectroscopy, pubmed-meshheading:11055720-Temperature
pubmed:year	2000
pubmed:articleTitle	Monitoring temperature changes in capillary electrophoresis with nanoliter-volume NMR thermometry.
pubmed:affiliation	Department of Chemistry, Electrical and Computer Engineering, the Beckman Institute, University of Illinois at Urbana-Champaign, 61801, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't