Source:http://linkedlifedata.com/resource/pubmed/id/11052817
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2000-12-18
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| pubmed:abstractText |
In order to investigate the role of the cytokine-induced neutrophil chemoattractant (CINC) in chronic bronchopulmonary infection, we developed a rat model of bronchopulmonary infection with Pseudomonas aeruginosa by using the agar bead method, and determined the kinetics of bacterial and cell number, as well as the concentrations of CINC-1, CINC-2, and CINC-3 in bronchoalveolar lavage (BAL) fluids in this model. The bacterial number in the lung rapidly increased from days 1 to 4, and declined 14 days after challenge. Neutrophil number in BAL fluid increased up to one day after challenge, and then slowly decreased during 14 days post-challenge. Among the CINCs, the local production of CINC-2 alpha sharply increased at day 1 and then decreased until day 4 post-challenge, while the local production of CINC-1 slightly increased at day 1 post-challenge. Neither CINC-2 beta nor CINC-3 were detected during the entire course of the infection. Increased CINC-2 mRNA expression in the lung tissue after challenge was associated with CINC-2 alpha production in BAL fluid. Moreover, an immunohistochemical study demonstrated the localization of CINC-1 and CINC-2 alpha primarily in alveolar macrophages and, to a much lesser extent, in bronchial epithelium of infected lung tissues, whereas CINC-2 beta and CINC-3 were not detected. When anti-CINC-1 or anti-CINC-2 alpha polyclonal antibodies were used for neutralizing neutrophil chemotactic activities in BAL fluids, the anti-CINC-2 alpha antibody inhibited 70% of the chemotactic activity in BAL fluids from infected rats at day 1 after challenge. No inhibition was observed by anti-CINC-1 antibody. These data indicate that CINC-2 alpha, which is produced by alveolar macrophages and bronchial epithelial cells, plays a pivotal role in neutrophil accumulation in the airway of a rat model of chronic bronchopulmonary infection with P. aeruginosa.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Gm1960 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1043-4666
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:volume |
12
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1662-8
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11052817-Animals,
pubmed-meshheading:11052817-Bronchi,
pubmed-meshheading:11052817-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:11052817-Cell Count,
pubmed-meshheading:11052817-Chemokine CXCL1,
pubmed-meshheading:11052817-Chemokines, CXC,
pubmed-meshheading:11052817-Chemotactic Factors,
pubmed-meshheading:11052817-Chemotaxis,
pubmed-meshheading:11052817-Chronic Disease,
pubmed-meshheading:11052817-Disease Models, Animal,
pubmed-meshheading:11052817-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11052817-Growth Substances,
pubmed-meshheading:11052817-Humans,
pubmed-meshheading:11052817-Immunohistochemistry,
pubmed-meshheading:11052817-Infection,
pubmed-meshheading:11052817-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11052817-Kinetics,
pubmed-meshheading:11052817-Lung,
pubmed-meshheading:11052817-Macrophages, Alveolar,
pubmed-meshheading:11052817-Neutrophils,
pubmed-meshheading:11052817-Pseudomonas aeruginosa,
pubmed-meshheading:11052817-RNA, Messenger,
pubmed-meshheading:11052817-Rabbits,
pubmed-meshheading:11052817-Rats,
pubmed-meshheading:11052817-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11052817-Time Factors
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| pubmed:year |
2000
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| pubmed:articleTitle |
Role of cytokine-induced neutrophil chemoattractant-2 (CINC-2) alpha in a rat model of chronic bronchopulmonary infections with Pseudomonas aeruginosa.
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| pubmed:affiliation |
Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Sakamoto, 1-12-4, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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