Source:http://linkedlifedata.com/resource/pubmed/id/11051263
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2001-1-29
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| pubmed:abstractText |
We have documented previously that adenovirus-mediated interleukin 12 (IL-12) gene therapy is effective for orthotopic tumor control and suppression of pre-established metastases in a preclinical prostate cancer model (Y. Nasu et al., Gene Ther., 6: 338-349, 1999). In this report, we directly compare the effectiveness of an adenovirus that expresses both IL-12 and the costimulatory molecule B7-1 (AdmIL12/B7) with one that expresses IL-12 alone (AdmIL-12) using the poorly immunogenic RM-9 orthotopic murine model of prostate cancer. We document AdmIL-12/B7-mediated secretion of IL-12 and increased surface expression of B7-1 in infected RM-9 tumor cells. A significant reduction in orthotopic tumor size and increased survival was demonstrated in mice treated with a single orthotopic injection of AdmIL-12/B7 compared with AdmIL-12 or controls. Six of 19 animals treated with AdmIL-12/B7 survived long term with apparent eradication of the primary tumor in contrast to one of 38 animals in the AdmIL-12-treated group. Orthotopic treatment of tumors with both vectors led to an infiltration of both CD4+ and CD8+ immunoreactive cells, with AdmIL-12/B7 treatment having a more prolonged infiltration of CD8+ cells. AdmIL-12/B7 was also more effective than AdmIL-12 or controls at suppression of pre-established metastases. We further developed a vaccine model based on s.c. injection of infected, irradiated RM-9 cells and found that both AdmIL-12 and AdmIL-12/B7 are effective at suppressing the development and growth of challenge orthotopic tumors using this protocol.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
|
| pubmed:issn |
1078-0432
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| pubmed:author |
pubmed-author:AlbanoDD,
pubmed-author:BangmaC HCH,
pubmed-author:EbaraSS,
pubmed-author:HullG WGW,
pubmed-author:LeeH MHM,
pubmed-author:MccurdyM AMA,
pubmed-author:NashDD,
pubmed-author:SatoTT,
pubmed-author:ShimuraSS,
pubmed-author:ThompsonT CTC,
pubmed-author:TimmeT LTL,
pubmed-author:WangJJ,
pubmed-author:YanoOO
|
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4101-9
|
| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11051263-Adenoviridae,
pubmed-meshheading:11051263-Animals,
pubmed-meshheading:11051263-Antigens, CD4,
pubmed-meshheading:11051263-Antigens, CD8,
pubmed-meshheading:11051263-Antigens, CD80,
pubmed-meshheading:11051263-Cancer Vaccines,
pubmed-meshheading:11051263-Flow Cytometry,
pubmed-meshheading:11051263-Gene Therapy,
pubmed-meshheading:11051263-Humans,
pubmed-meshheading:11051263-Immunohistochemistry,
pubmed-meshheading:11051263-Interleukin-12,
pubmed-meshheading:11051263-Kinetics,
pubmed-meshheading:11051263-Lung Neoplasms,
pubmed-meshheading:11051263-Male,
pubmed-meshheading:11051263-Mice,
pubmed-meshheading:11051263-Mice, Inbred C57BL,
pubmed-meshheading:11051263-Neoplasm Transplantation,
pubmed-meshheading:11051263-Prostatic Neoplasms,
pubmed-meshheading:11051263-Time Factors,
pubmed-meshheading:11051263-Tumor Cells, Cultured
|
| pubmed:year |
2000
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| pubmed:articleTitle |
Prostate cancer gene therapy: comparison of adenovirus-mediated expression of interleukin 12 with interleukin 12 plus B7-1 for in situ gene therapy and gene-modified, cell-based vaccines.
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| pubmed:affiliation |
Scott Department of Urology Baylor College of Medicine, Houston, Texas 77030, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|