Linked Life Data

11051223

Source:http://linkedlifedata.com/resource/pubmed/id/11051223

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2001-1-29
pubmed:abstractText
Mutations of the von Hippel-Lindau (VHL) tumor suppressor gene have been detected in up to 60% of sporadic clear cell renal carcinomas (RCCs). Even patients with RCCs believed to be curable with radical nephrectomy sometimes develop distant metastasis 5-10 years after surgery, suggesting hematogenous circulation of cancer cells. Useful tumor markers have not yet been established for RCC. To detect patients at high risk of metastasis after surgery, we developed a highly sensitive and specific nested reverse transcription-PCR method using VHL gene mutation to detect circulating cancer cells. We screened 29 sporadic clear cell RCCs from patients for mutations of the VHL gene by direct sequencing. We next examined blood samples from patients with the VHL gene mutation using mutation-specific nested reverse transcription-PCR. Somatic mutations were detected in 20 of 29 (69.0%) sporadic clear cell RCCs. The VHL gene mutations were detected in peripheral and/or renal venous blood from 15 of 20 (75%) patients. The mutations were detected in the peripheral blood in 2 of 17 (11.8%) patients before surgery, 6 of 16 (37.5%) patients within 24 h after surgery, 3 of 16 (18.8%) patients on day 7 after surgery, and 2 of 11 (18.2%) patients on day 30 after surgery. In seven of nine (77.8%) patients, mutations were detected in renal venous blood during surgery. These findings indicate the presence of circulating cancer cells with VHL gene mutation. Although much larger studies are needed to determine the clinical significance, our study shows that this technique is feasible for detecting circulating RCC cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1078-0432
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3817-22
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11051223-Adult, pubmed-meshheading:11051223-Aged, pubmed-meshheading:11051223-Aged, 80 and over, pubmed-meshheading:11051223-Alleles, pubmed-meshheading:11051223-Carcinoma, Renal Cell, pubmed-meshheading:11051223-DNA, Complementary, pubmed-meshheading:11051223-Female, pubmed-meshheading:11051223-Humans, pubmed-meshheading:11051223-Kidney Neoplasms, pubmed-meshheading:11051223-Ligases, pubmed-meshheading:11051223-Male, pubmed-meshheading:11051223-Middle Aged, pubmed-meshheading:11051223-Models, Genetic, pubmed-meshheading:11051223-Mutation, pubmed-meshheading:11051223-Neoplastic Cells, Circulating, pubmed-meshheading:11051223-Protein Biosynthesis, pubmed-meshheading:11051223-Proteins, pubmed-meshheading:11051223-RNA, pubmed-meshheading:11051223-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11051223-Risk, pubmed-meshheading:11051223-Sensitivity and Specificity, pubmed-meshheading:11051223-Sequence Analysis, DNA, pubmed-meshheading:11051223-Time Factors, pubmed-meshheading:11051223-Tumor Suppressor Proteins, pubmed-meshheading:11051223-Ubiquitin-Protein Ligases, pubmed-meshheading:11051223-Von Hippel-Lindau Tumor Suppressor Protein
pubmed:year
2000
pubmed:articleTitle
Detection of circulating cancer cells with von hippel-lindau gene mutation in peripheral blood of patients with renal cell carcinoma.
pubmed:affiliation
Department of Urology, Kochi Medical School, Kochi, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't