Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2000-11-27
pubmed:abstractText
Dopamine and cAMP-regulated phosphoprotein of M(r) 32,000 (DARPP-32) plays an obligatory role in most of the actions of dopamine. In resting neostriatal slices, cyclin-dependent kinase 5 (Cdk5) phosphorylates DARPP-32 at Thr-75, thereby reducing the efficacy of dopaminergic signaling. We report here that dopamine, in slices, and acute cocaine, in whole animals, decreases the state of phosphorylation of striatal DARPP-32 at Thr-75 and thereby removes this inhibitory constraint. This effect of dopamine is achieved through dopamine D1 receptor-mediated activation of cAMP-dependent protein kinase (PKA). The activated PKA, by decreasing the state of phosphorylation of DARPP-32-Thr-75, de-inhibits itself. Dopamine D2 receptor stimulation has the opposite effect. The ability of activated PKA to reduce the state of phosphorylation of DARPP-32-Thr-75 is apparently attributable to increased protein phosphatase-2A activity, with Cdk5 being unaffected. Together, these results indicate that via positive feedback mechanisms, Cdk5 signaling and PKA signaling are mutually antagonistic.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10217279, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10395578, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10433257, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10500146, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10604467, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10604473, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-10896159, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-1494946, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-2539153, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-2831201, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-3711991, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-6087160, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-6273735, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-6296332, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-6319627, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-7592815, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-7760048, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-8306873, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-8694763, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-8703017, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-8939471, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-8940115, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-8982166, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-9108010, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-9276686, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-9334390, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-9688562, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-9694658, http://linkedlifedata.com/resource/pubmed/commentcorrection/11050161-9727024
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Calcineurin, http://linkedlifedata.com/resource/pubmed/chemical/Cdk5 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Cocaine, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 5, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Dopamine and cAMP-Regulated..., http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 2, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D1, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D2
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12840-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11050161-Animals, pubmed-meshheading:11050161-Calcineurin, pubmed-meshheading:11050161-Cocaine, pubmed-meshheading:11050161-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:11050161-Cyclin-Dependent Kinase 5, pubmed-meshheading:11050161-Cyclin-Dependent Kinases, pubmed-meshheading:11050161-Dopamine, pubmed-meshheading:11050161-Dopamine and cAMP-Regulated Phosphoprotein 32, pubmed-meshheading:11050161-Feedback, pubmed-meshheading:11050161-Mice, pubmed-meshheading:11050161-Mice, Inbred C57BL, pubmed-meshheading:11050161-Neostriatum, pubmed-meshheading:11050161-Nerve Tissue Proteins, pubmed-meshheading:11050161-Phosphoprotein Phosphatases, pubmed-meshheading:11050161-Phosphoproteins, pubmed-meshheading:11050161-Phosphorylation, pubmed-meshheading:11050161-Protein Phosphatase 2, pubmed-meshheading:11050161-Receptors, Dopamine D1, pubmed-meshheading:11050161-Receptors, Dopamine D2, pubmed-meshheading:11050161-Signal Transduction
pubmed:year
2000
pubmed:articleTitle
Amplification of dopaminergic signaling by a positive feedback loop.
pubmed:affiliation
Department of Physiology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan. nishia@med.kurume-u.ac.jp
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.
More...