Linked Life Data

11049988

Source:http://linkedlifedata.com/resource/pubmed/id/11049988

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2000-12-11
pubmed:abstractText
We identified antibacterial components in human T and natural killer (NK) cells by using freshly isolated lymphocytes enriched for T and NK cells as starting material. After growing these lymphocytes for 5 days in the presence of interleukin (IL)-2, we isolated and characterized several antibacterial peptides/proteins from the supernatant-alpha-defensins (HNP 1-3), LL-37, lysozyme, and a fragment of histone H2B-although other active components were also present. We then used reverse transcriptase-polymerase chain reaction to search for expression of the gene coding for LL-37 in several B-cell lines, gammadelta T-cell lines, NK clones, and one monocytic cell line, with positive results, but found no expression in several alphabeta T-cell lines. The alpha-defensins (HNP 1-3) were also found to be expressed in several of these cell lines. To confirm the presence of these antibacterial peptides in lymphocytes, we localized them to NK, gammadelta T cells, B cells, and monocytes/macrophages by using double-staining immunohistochemical analysis of freshly isolated lymphocytes. We also found that primary cultures of lymphocytes transcribe and secrete LL-37 and that these processes are affected by IL-6 and interferon-gamma. In addition, we demonstrated that LL-37 has chemotactic activity for polymorphonuclear leukocytes and CD4 T lymphocytes, whereas others have shown chemotactic activity for human alpha-defensins (HNP 1-2). These findings suggest that microbicidal peptides are effector molecules of lymphocytes and that antibacterial activity previously shown to be derived from T and NK cells may be partly mediated by the antibacterial peptides LL-37 and HNP 1-3.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3086-93
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11049988-Anti-Bacterial Agents, pubmed-meshheading:11049988-Antimicrobial Cationic Peptides, pubmed-meshheading:11049988-B-Lymphocytes, pubmed-meshheading:11049988-Carrier Proteins, pubmed-meshheading:11049988-Cathelicidins, pubmed-meshheading:11049988-Cell Line, pubmed-meshheading:11049988-Chemotaxis, Leukocyte, pubmed-meshheading:11049988-Cloning, Molecular, pubmed-meshheading:11049988-Histones, pubmed-meshheading:11049988-Humans, pubmed-meshheading:11049988-Immunohistochemistry, pubmed-meshheading:11049988-Interferon-gamma, pubmed-meshheading:11049988-Interleukin-6, pubmed-meshheading:11049988-Killer Cells, Natural, pubmed-meshheading:11049988-Lymphocyte Activation, pubmed-meshheading:11049988-Lymphocytes, pubmed-meshheading:11049988-Monocytes, pubmed-meshheading:11049988-Muramidase, pubmed-meshheading:11049988-Neutrophils, pubmed-meshheading:11049988-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11049988-T-Lymphocytes, pubmed-meshheading:11049988-alpha-Defensins
pubmed:year
2000
pubmed:articleTitle
The human antimicrobial and chemotactic peptides LL-37 and alpha-defensins are expressed by specific lymphocyte and monocyte populations.
pubmed:affiliation
Department of Medical Biochemistry and Biophysics, Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't