|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2001-1-3
|
|
pubmed:abstractText |
We investigated the ability of signal peptides of eukaryotic origin (human, mouse, and yeast) to efficiently direct model proteins to the Escherichia coli periplasm. These were compared against a well-characterized prokaryotic signal peptide-OmpA. Surprisingly, eukaryotic signal peptides can work very efficiently in E. coli, but require optimization of codon usage by codon-based mutagenesis of the signal peptide coding region. Analysis of the 5' of periplasmic and cytoplasmic E. coli genes shows some codon usage differences.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
1046-5928
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2000 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
20
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
252-64
|
|
pubmed:dateRevised |
2006-5-1
|
|
pubmed:meshHeading |
pubmed-meshheading:11049749-Amino Acid Sequence,
pubmed-meshheading:11049749-Animals,
pubmed-meshheading:11049749-Base Sequence,
pubmed-meshheading:11049749-Cloning, Molecular,
pubmed-meshheading:11049749-Codon,
pubmed-meshheading:11049749-Databases, Factual,
pubmed-meshheading:11049749-Escherichia coli,
pubmed-meshheading:11049749-Gene Expression Regulation, Bacterial,
pubmed-meshheading:11049749-Genes, Bacterial,
pubmed-meshheading:11049749-Genetic Code,
pubmed-meshheading:11049749-Humans,
pubmed-meshheading:11049749-Immunoglobulin Fab Fragments,
pubmed-meshheading:11049749-Mice,
pubmed-meshheading:11049749-Molecular Sequence Data,
pubmed-meshheading:11049749-Mutagenesis,
pubmed-meshheading:11049749-Nucleic Acid Conformation,
pubmed-meshheading:11049749-Periplasm,
pubmed-meshheading:11049749-Plasmids,
pubmed-meshheading:11049749-Protein Sorting Signals,
pubmed-meshheading:11049749-Protein Transport,
pubmed-meshheading:11049749-RNA, Messenger,
pubmed-meshheading:11049749-RNA Stability,
pubmed-meshheading:11049749-Sequence Analysis, Protein,
pubmed-meshheading:11049749-Yeasts
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5' end of the coding sequence.
|
|
pubmed:affiliation |
Celltech-Chiroscience, 216 Bath Road, Slough, Berkshire, SL1 4EN, United Kingdom. dhumphre@celltech.co.uk
|
|
pubmed:publicationType |
Journal Article
|
