Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2000-11-3
pubmed:abstractText
In contrast to extensive studies on the role of T and B lymphocytes in the pathogenesis of autoimmune diseases of the nervous system, little is known about NK cells and their potential role in the destruction of neural tissue. NK cells have been implicated in the selective death of sympathetic neurons resident in the superior cervical ganglia of rats after exposure to the drug guanethidine. This observation suggests that NK cells may function as principle effectors in immunological diseases of the nervous system. However, the direct mechanism of action of NK cells in this model is not known. In particular, it is not known whether NK cells can kill autologous neurons directly. The aim of the present study was to examine whether NK cells can kill directly dorsal root ganglia neurons cultured in vitro. We demonstrate that C57BL/6 (B6)-derived dorsal root ganglia neurons can be killed directly by syngenic IL-2-activated NK cells, and that this nerve cell lysis is dependent on the expression of perforin in the NK cells. NK cells were less effective in destroying neurons grown in the presence of glial cells. These observations indicate a potential role for NK cells in nerve cell degeneration in inflammatory diseases of the nervous system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4895-900
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11046014-Animals, pubmed-meshheading:11046014-Cell Communication, pubmed-meshheading:11046014-Cell Count, pubmed-meshheading:11046014-Cells, Cultured, pubmed-meshheading:11046014-Cytotoxicity, Immunologic, pubmed-meshheading:11046014-Cytotoxicity Tests, Immunologic, pubmed-meshheading:11046014-Ganglia, Spinal, pubmed-meshheading:11046014-Killer Cells, Natural, pubmed-meshheading:11046014-Male, pubmed-meshheading:11046014-Membrane Glycoproteins, pubmed-meshheading:11046014-Mice, pubmed-meshheading:11046014-Mice, Inbred C57BL, pubmed-meshheading:11046014-Mice, Knockout, pubmed-meshheading:11046014-Nerve Degeneration, pubmed-meshheading:11046014-Neuroglia, pubmed-meshheading:11046014-Neurons, pubmed-meshheading:11046014-Perforin, pubmed-meshheading:11046014-Pore Forming Cytotoxic Proteins
pubmed:year
2000
pubmed:articleTitle
Direct NK cell-mediated lysis of syngenic dorsal root ganglia neurons in vitro.
pubmed:affiliation
Department of Neuroscience, and Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden. eva.backstrom@neuro.ki.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't