|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
22
|
|
pubmed:dateCreated |
2000-11-21
|
|
pubmed:abstractText |
The carboxy-terminal region of the rabbit hemorrhagic disease virus p37 polyprotein cleavage product has been expressed in Escherichia coli as a glutathione S-transferase (GST) fusion protein. The recombinant GST-Delta2C protein showed in vitro ATP-binding and ATPase activities. Site-directed mutagenesis studies of the conserved residues G(522) and T(529) in motif A, D(566) and E(567) in motif B, and K(600) in motif C were also performed. These results provide the first experimental characterization of a 2C-like ATPase activity in a member of the Caliciviridae.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-10066753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-10074162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1322588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1322957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1378397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1716026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1731253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1760582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-1840711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-2156730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-2164609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-2196171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-2352325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-6329717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-7474137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-7925384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-8116241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-8380474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-8385138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-8396675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-8551592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-8892921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9060609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9311795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9525622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9557747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9614113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9645199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9658124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11044135-9742051
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
74
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
10846-51
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11044135-Adenosine Triphosphatases,
pubmed-meshheading:11044135-Adenosine Triphosphate,
pubmed-meshheading:11044135-Animals,
pubmed-meshheading:11044135-Carrier Proteins,
pubmed-meshheading:11044135-Glutathione Transferase,
pubmed-meshheading:11044135-Hemorrhagic Disease Virus, Rabbit,
pubmed-meshheading:11044135-Mutagenesis, Site-Directed,
pubmed-meshheading:11044135-Rabbits,
pubmed-meshheading:11044135-Recombinant Fusion Proteins,
pubmed-meshheading:11044135-Viral Nonstructural Proteins
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
ATP binding and ATPase activities associated with recombinant rabbit hemorrhagic disease virus 2C-like polypeptide.
|
|
pubmed:affiliation |
Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias (CSIC), Universidad de Oviedo, 33006 Oviedo, Spain.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
