Linked Life Data

11043467

Source:http://linkedlifedata.com/resource/pubmed/id/11043467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2001-1-11
pubmed:databankReference
pubmed:abstractText
Two cDNA clones were isolated from soybean (Glycine soja) by polymerase chain reaction with primers designed to conserved motifs found in apyrases (nucleotide phosphohydrolase). The two cDNAs are predicted to encode for two, distinct, apyrase proteins of approximately 50 kDa (i.e., GS50) and 52 kDa (i.e., GS52). Phylogenetic analysis indicated that GS52 is orthologous to a family of apyrases recently suggested to play a role in legume nodulation. GS50 is paralogous to this family and, therefore, likely plays a different physiological role. Consistent with this analysis, GS50 mRNA was detected in root, hypocotyls, flowers, and stems, while GS52 mRNA was found in root and flowers. Neither gene was expressed in leaves or cotyledons. Inoculation of roots with Bradyrhizobium japonicum, nitrogen-fixing symbiont of soybean, resulted in the rapid (<6 h) induction of GS52 mRNA expression. The level of GS50 mRNA expression was not affected by bacterial inoculation. Western blot (immunoblot) analysis of GS50 expression mirrored the results obtained by mRNA analysis. However, in contrast to the mRNA results, GS52 protein was found in stems. Interestingly, anti-GS52 antibody recognized a 50-kDa protein found only in nodule extracts. Treatment of roots with anti-GS52 antibody, but not anti-GS50 antibody or preimmune serum, blocked nodulation by B. japonicum. Fractionation of cellular membranes in sucrose density gradients and subsequent Western analysis of the fractions revealed that GS50 colocalized with marker enzymes for the Golgi, while GS52 colocalized with marker enzymes for the plasma membrane. Restriction fragment length polymorphism (RFLP)-based mapping placed the gs52 gene on major linkage group J of the integrated genetic map of soybean. These data suggest that GS50 is likely an endo-apyrase involved in Golgi function, while GS52 is localized on the root surface and appears to play an important role in nodulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0894-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1053-70
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11043467-Amino Acid Sequence, pubmed-meshheading:11043467-Antibodies, pubmed-meshheading:11043467-Apyrase, pubmed-meshheading:11043467-Bradyrhizobium, pubmed-meshheading:11043467-Chromosome Mapping, pubmed-meshheading:11043467-DNA, Complementary, pubmed-meshheading:11043467-DNA Primers, pubmed-meshheading:11043467-Gene Expression Regulation, Plant, pubmed-meshheading:11043467-Genes, Plant, pubmed-meshheading:11043467-Membrane Proteins, pubmed-meshheading:11043467-Molecular Sequence Data, pubmed-meshheading:11043467-Nucleic Acid Hybridization, pubmed-meshheading:11043467-Plant Proteins, pubmed-meshheading:11043467-Plant Roots, pubmed-meshheading:11043467-Plant Structures, pubmed-meshheading:11043467-Polymerase Chain Reaction, pubmed-meshheading:11043467-RNA, Messenger, pubmed-meshheading:11043467-RNA, Plant, pubmed-meshheading:11043467-Recombinant Fusion Proteins, pubmed-meshheading:11043467-Soybeans
pubmed:year
2000
pubmed:articleTitle
Differential expression of two soybean apyrases, one of which is an early nodulin.
pubmed:affiliation
Center for Legume Research, Department of Microbiology, University of Tennessee, Knoxville 37996, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.