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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0040649,
umls-concept:C0079904,
umls-concept:C0132555,
umls-concept:C0205263,
umls-concept:C0205314,
umls-concept:C0243042,
umls-concept:C0679622,
umls-concept:C0851285,
umls-concept:C1366763,
umls-concept:C1706322
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pubmed:issue |
5
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pubmed:dateCreated |
2001-5-23
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pubmed:abstractText |
Inflammation is a hallmark of several vascular diseases. The nuclear factor kappaB (NF-kappaB) transcription factors are dimeric proteins involved in the activation of a large number of genes in response to inflammatory stimuli. We report the involvement of a novel member of the ETS transcription factor, ESE-1, in mediating vascular inflammation. ESE-1 is induced in response to inflammatory cytokines and lipopolysaccharide in vascular smooth muscle cells, endothelial cells, and cells of the monocyte-macrophage lineage. This induction occurs within hours of stimulation and is mediated by NF-kappaB transactivation of the ESE-1 promoter. We have identified the inducible form of nitric-oxide synthase (NOS2) as a putative target for ESE-1. ESE-1 can bind to the p50 subunit of NF-kappaB, and cotransfection of ESE-1 with the p50 and p65 subunits of NF-kappaB synergistically enhances transactivation of the NOS2 promoter by ESE-1. An ESE-1-binding site within the NOS2 promoter has been identified, the site-directed mutagenesis of which completely abolishes the ability of ESE-1 to transactivate the NOS2 promoter. Finally, in a mouse model of endotoxemia, associated with acute vascular inflammation, ESE-1 is strongly expressed in vascular endothelium and smooth muscle cells. In summary, ESE-1 represents a novel mediator of vascular inflammation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ELF3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NOS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmin I,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3302-9
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pubmed:dateRevised |
2011-10-27
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pubmed:meshHeading |
pubmed-meshheading:11036073-Binding Sites,
pubmed-meshheading:11036073-Calcium-Binding Proteins,
pubmed-meshheading:11036073-Cells, Cultured,
pubmed-meshheading:11036073-Cytokines,
pubmed-meshheading:11036073-DNA-Binding Proteins,
pubmed-meshheading:11036073-Humans,
pubmed-meshheading:11036073-Inflammation,
pubmed-meshheading:11036073-Inflammation Mediators,
pubmed-meshheading:11036073-Membrane Glycoproteins,
pubmed-meshheading:11036073-Mutation,
pubmed-meshheading:11036073-NF-kappa B,
pubmed-meshheading:11036073-Nerve Tissue Proteins,
pubmed-meshheading:11036073-Nitric Oxide Synthase,
pubmed-meshheading:11036073-Nitric Oxide Synthase Type II,
pubmed-meshheading:11036073-Promoter Regions, Genetic,
pubmed-meshheading:11036073-Protein Structure, Tertiary,
pubmed-meshheading:11036073-Proto-Oncogene Proteins,
pubmed-meshheading:11036073-Synaptotagmin I,
pubmed-meshheading:11036073-Synaptotagmins,
pubmed-meshheading:11036073-Trans-Activators,
pubmed-meshheading:11036073-Transcription Factors,
pubmed-meshheading:11036073-Transcriptional Activation,
pubmed-meshheading:11036073-Vascular Diseases
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pubmed:year |
2001
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pubmed:articleTitle |
ESE-1 is a novel transcriptional mediator of inflammation that interacts with NF-kappa B to regulate the inducible nitric-oxide synthase gene.
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pubmed:affiliation |
Cardiology Division, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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