rdf:type |
|
lifeskim:mentions |
umls-concept:C0000843,
umls-concept:C0006675,
umls-concept:C0007603,
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0030971,
umls-concept:C0162740,
umls-concept:C0205224,
umls-concept:C1382107,
umls-concept:C1422505,
umls-concept:C1444748
|
pubmed:issue |
1
|
pubmed:dateCreated |
2000-11-7
|
pubmed:abstractText |
Pretreatment of Arabidopsis thaliana suspension cells with impermeant calcium chelator EGTA inhibited the ABA-induced RAB18 gene expression. However, extracellular calcium alone, up to 10 mM, did not trigger RAB18 expression. Spectrofluorimetric extracellular Ca(2+) measurement with Fluo-3 showed a fast, within 1 min, Ca(2+) influx associated with outer plasmalemma ABA perception. In the presence of the Ca(2+) blockers Cd(2+) and Ni(2+), RAB18 expression was suppressed. Pimozide and fluspirilene inhibited Ca(2+) influx and ABA-induced RAB18 expression. Thus we demonstrated the involvement of specific Ca(2+) influx in the ABA signaling sequence leading to RAB18 expression.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0014-5793
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
13
|
pubmed:volume |
483
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
67-70
|
pubmed:dateRevised |
2003-11-14
|
pubmed:meshHeading |
pubmed-meshheading:11033358-Abscisic Acid,
pubmed-meshheading:11033358-Arabidopsis,
pubmed-meshheading:11033358-Arabidopsis Proteins,
pubmed-meshheading:11033358-Biological Transport,
pubmed-meshheading:11033358-Blotting, Northern,
pubmed-meshheading:11033358-Calcium,
pubmed-meshheading:11033358-Cell Membrane,
pubmed-meshheading:11033358-Dose-Response Relationship, Drug,
pubmed-meshheading:11033358-Extracellular Space,
pubmed-meshheading:11033358-Gene Expression Regulation, Plant,
pubmed-meshheading:11033358-Plant Proteins,
pubmed-meshheading:11033358-RNA, Plant,
pubmed-meshheading:11033358-Time Factors,
pubmed-meshheading:11033358-rab GTP-Binding Proteins
|
pubmed:year |
2000
|
pubmed:articleTitle |
Abscisic acid plasmalemma perception triggers a calcium influx essential for RAB18 gene expression in Arabidopsis thaliana suspension cells.
|
pubmed:affiliation |
Physiologie Cellulaire et Moléculaire des Plantes, UMR CNRS 7632, case 156, Université Pierre et Marie Curie (Paris VI), 4 place Jussieu, 75252 Cedex 05, Paris, France.
|
pubmed:publicationType |
Journal Article
|