Source:http://linkedlifedata.com/resource/pubmed/id/11032749
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-11-6
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| pubmed:databankReference | |
| pubmed:abstractText |
We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus ( approximately 24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical ( approximately 72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 ( approximately 24% and approximately 22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. Therefore, FGF-23 is expected a unique FGF that plays roles in the function of the ventrolateral thalamic nucleus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/FGF19 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fibroblast growth factor 21
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0006-291X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:day |
22
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| pubmed:volume |
277
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
494-8
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11032749-Amino Acid Sequence,
pubmed-meshheading:11032749-Animals,
pubmed-meshheading:11032749-Baculoviridae,
pubmed-meshheading:11032749-Blotting, Western,
pubmed-meshheading:11032749-Brain,
pubmed-meshheading:11032749-Cell Line,
pubmed-meshheading:11032749-Chromosome Mapping,
pubmed-meshheading:11032749-Chromosomes, Human, Pair 12,
pubmed-meshheading:11032749-DNA, Complementary,
pubmed-meshheading:11032749-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11032749-Fibroblast Growth Factors,
pubmed-meshheading:11032749-Humans,
pubmed-meshheading:11032749-In Situ Hybridization,
pubmed-meshheading:11032749-Mice,
pubmed-meshheading:11032749-Molecular Sequence Data,
pubmed-meshheading:11032749-Phylogeny,
pubmed-meshheading:11032749-RNA, Complementary,
pubmed-meshheading:11032749-RNA, Messenger,
pubmed-meshheading:11032749-Recombinant Proteins,
pubmed-meshheading:11032749-Sequence Homology, Amino Acid,
pubmed-meshheading:11032749-Tissue Distribution,
pubmed-meshheading:11032749-Ventral Thalamic Nuclei
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| pubmed:year |
2000
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| pubmed:articleTitle |
Identification of a novel fibroblast growth factor, FGF-23, preferentially expressed in the ventrolateral thalamic nucleus of the brain.
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| pubmed:affiliation |
Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Yoshida-Shimoadachi, Sakyo, Kyoto, 606-8501, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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