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rdf:type |
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lifeskim:mentions |
umls-concept:C0018270,
umls-concept:C0019932,
umls-concept:C0022942,
umls-concept:C0024660,
umls-concept:C0027912,
umls-concept:C0028351,
umls-concept:C0038848,
umls-concept:C0040679,
umls-concept:C0150312,
umls-concept:C0302583,
umls-concept:C0521009
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pubmed:issue |
21
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pubmed:dateCreated |
2000-11-24
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pubmed:abstractText |
Norepinephrine stimulates the growth of a range of bacterial species in nutritionally poor SAPI minimal salts medium containing 30% serum. Addition of size-fractionated serum components to SAPI medium indicated that transferrin was required for norepinephrine stimulation of growth of Escherichia coli. Since bacteriostasis by serum is primarily due to the iron-withholding capacity of transferrin, we considered the possibility that norepinephrine can overcome this effect by supplying transferrin-bound iron for growth. Incubation with concentrations of norepinephrine that stimulated bacterial growth in serum-SAPI medium resulted in loss of bound iron from iron-saturated transferrin, as indicated by the appearance of monoferric and apo- isoforms upon electrophoresis in denaturing gels. Norepinephrine also caused the loss of iron from lactoferrin. The pharmacologically inactive metabolite norepinephrine 3-O-sulfate, by contrast, did not result in iron loss from transferrin or lactoferrin and did not stimulate bacterial growth in serum-SAPI medium. Norepinephrine formed stable complexes with transferrin, lactoferrin, and serum albumin. Norepinephrine-transferrin and norepinephrine-lactoferrin complexes, but not norepinephrine-apotransferrin or norepinephrine-albumin complexes, stimulated bacterial growth in serum-SAPI medium in the absence of additional norepinephrine. Norepinephrine-stimulated growth in medium containing (55)Fe complexed with transferrin or lactoferrin resulted in uptake of radioactivity by bacterial cells. Moreover, norepinephrine-stimulated growth in medium containing [(3)H]norepinephrine indicated concomitant uptake of norepinephrine. In each case, addition of excess iron did not affect growth but significantly reduced levels of radioactivity ((55)Fe or (3)H) associated with bacterial cells. A role for catecholamine-mediated iron supply in the pathophysiology of infectious diseases is proposed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-10079527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-10355615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-1385631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-1585458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-1642533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-2974803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-3341911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-3889357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-4717703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-5571840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-6188699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-6298936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-6455965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-7691742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-7826025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8026441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8268005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8371072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8381276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8627312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8666202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8739412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8818707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-8833888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9105222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9126336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9169764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9226263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9245571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9634294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-9712730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11029429-999884
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6091-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
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pubmed:year |
2000
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pubmed:articleTitle |
The mammalian neuroendocrine hormone norepinephrine supplies iron for bacterial growth in the presence of transferrin or lactoferrin.
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pubmed:affiliation |
Department of Microbiology & Immunology, University of Leicester, Leicester LE1 9HN, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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