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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2000-11-6
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pubmed:abstractText |
The GATA-6 transcription factor is reported to be expressed in vascular myocytes. Because glomerular mesangial cells (GMCs) and vascular myocytes have similar properties, we examined whether GATA-6 was expressed in cultured GMCs and whether overexpression of GATA-6 induced cell cycle arrest in GMCs, using a recombinant adenovirus that expresses GATA-6 (Ad GATA-6). GATA-6 expression in GMCs was downregulated when quiescent GMCs were stimulated by serum to reenter the cell cycle. [(3)H]thymidine uptake was inhibited in GMCs infected with Ad GATA-6 in a dose- and time-dependent manner. The expression of cyclin A protein was decreased and that of the cyclin-dependent kinase inhibitor p21(cip1) was increased in GMCs infected with Ad GATA-6. Although the expression of p21(cip1) transcripts did not change remarkably, p21(cip1) protein was stabilized in GMCs infected with Ad GATA-6, suggesting a post-transcriptional regulation of p21(cip1) expression. Northern blot analysis showed that expression of the cyclin A transcript was decreased in Ad GATA-6-infected cells, whereas this decrease of cyclin A was not observed in GMCs derived from p21(cip1) null mice. Our results demonstrate that GATA-6 is endogenously expressed in GMCs and that overexpression of GATA-6 can induce cell cycle arrest. Our results also show that GATA-6-induced cell cycle arrest is associated with inhibition of cyclin A expression and p21(cip1) upregulation. Finally, our results indicate that the GATA-6-induced suppression of cyclin A expression depends on the presence of p21(cip1).
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin A,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1524-4571
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pubmed:author |
pubmed-author:GotoAA,
pubmed-author:HirataYY,
pubmed-author:KakokiMM,
pubmed-author:ManiNN,
pubmed-author:NagataDD,
pubmed-author:NishimatsuHH,
pubmed-author:OmataMM,
pubmed-author:SataMM,
pubmed-author:SuzukiEE,
pubmed-author:WalshKK,
pubmed-author:YoshizumiMM
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pubmed:issnType |
Electronic
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pubmed:day |
13
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
699-704
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11029406-Adenoviridae,
pubmed-meshheading:11029406-Animals,
pubmed-meshheading:11029406-Blood Proteins,
pubmed-meshheading:11029406-Blotting, Northern,
pubmed-meshheading:11029406-CDC2-CDC28 Kinases,
pubmed-meshheading:11029406-Cell Cycle,
pubmed-meshheading:11029406-Cell Cycle Proteins,
pubmed-meshheading:11029406-Cell Division,
pubmed-meshheading:11029406-Cells, Cultured,
pubmed-meshheading:11029406-Cyclin A,
pubmed-meshheading:11029406-Cyclin-Dependent Kinase 2,
pubmed-meshheading:11029406-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:11029406-Cyclin-Dependent Kinases,
pubmed-meshheading:11029406-Cyclins,
pubmed-meshheading:11029406-DNA-Binding Proteins,
pubmed-meshheading:11029406-Dose-Response Relationship, Drug,
pubmed-meshheading:11029406-Down-Regulation,
pubmed-meshheading:11029406-GATA6 Transcription Factor,
pubmed-meshheading:11029406-Gene Expression,
pubmed-meshheading:11029406-Glomerular Mesangium,
pubmed-meshheading:11029406-Humans,
pubmed-meshheading:11029406-Muscle, Smooth, Vascular,
pubmed-meshheading:11029406-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11029406-RNA, Messenger,
pubmed-meshheading:11029406-Rats,
pubmed-meshheading:11029406-Recombinant Proteins,
pubmed-meshheading:11029406-Thymidine,
pubmed-meshheading:11029406-Transcription Factors,
pubmed-meshheading:11029406-Up-Regulation
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pubmed:year |
2000
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pubmed:articleTitle |
Cyclin A downregulation and p21(cip1) upregulation correlate with GATA-6-induced growth arrest in glomerular mesangial cells.
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pubmed:affiliation |
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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