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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-10-30
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pubmed:abstractText |
LEDGF promotes survival of many cell types against a wide range of environmental stresses, and cells responding to those stresses expressed higher levels of heat shock proteins (Hsps). LEDGF/p75 is a weak coactivator of general transcription. We speculated that a stress signal may activate expression of the LEDGF gene, resulting in elevated levels of LEDGF which may activate expression of stress-related proteins to protect cells from stresses. Lens epithelial cells (LECs) and cos7 cells were cultured under heat- or oxidative-stress. After cells were cultured for defined time, we quantified LEDGF mRNA and LEDGF protein with RT-PCR, Northern blot, and protein blot analysis. Our results showed that higher levels of LEDGF were found in both cell types under heat- and oxidative-stress than that in cells cultured at nonstress condition. Thus, one of the primary events in the stress-related protective events is the activation of LEDGF, a regulatory protein.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1320-4
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11027629-Animals,
pubmed-meshheading:11027629-Blotting, Northern,
pubmed-meshheading:11027629-Blotting, Western,
pubmed-meshheading:11027629-COS Cells,
pubmed-meshheading:11027629-Epithelial Cells,
pubmed-meshheading:11027629-Growth Substances,
pubmed-meshheading:11027629-Heat-Shock Response,
pubmed-meshheading:11027629-Hot Temperature,
pubmed-meshheading:11027629-Hydrogen Peroxide,
pubmed-meshheading:11027629-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11027629-Lens, Crystalline,
pubmed-meshheading:11027629-Mice,
pubmed-meshheading:11027629-Oxidative Stress,
pubmed-meshheading:11027629-RNA, Messenger,
pubmed-meshheading:11027629-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11027629-Up-Regulation
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pubmed:year |
2000
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pubmed:articleTitle |
Activation of LEDGF gene by thermal-and oxidative-stresses.
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pubmed:affiliation |
Center for Ophthalmic Research, Department of Ophthalmology, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Avenue, Boston, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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