Source:http://linkedlifedata.com/resource/pubmed/id/11027578
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2000-10-30
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| pubmed:databankReference | |
| pubmed:abstractText |
We isolated and characterized a murine orthologue of PTP-BK, a receptor-type protein tyrosine phosphatase expressed in brain and kidney. The deduced amino acid sequences showed 89, 95, and 92% identities with human, rabbit, and chicken homologues of PTP-BK. Mouse PTP-BK encodes a polypeptide of 1,226 amino acids with calculated molecular weight of 138,598 Da. The mature form of PTP-BK constitutes of 3 domain structures including extracellular, transmembrane, and a single intracellular PTPase domain. Western blot analysis indicated that anti-PTP-BK antibody specifically immunoreacted to 2 distinct molecules of 200 kDa and 220 kDa in COS cells, possibly due to differential glycosylation. The recombinant PTP-BK showed the phosphatase activity specific for the phosphotyrosine, but not for the phosphoserine residue of phosphopeptides in vitro. Radiation hybrid panel assigned mouse PTP-BK gene to 5.02cR distal to from the marker D6Mit339 on chromosome 6. Mouse PTP-BK was classified in PTPRO family in novel nomenclature. We discuss here the diversity and physiological functions of PTPRO family of PTP.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0006-291X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:day |
5
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| pubmed:volume |
276
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
974-81
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11027578-Amino Acid Sequence,
pubmed-meshheading:11027578-Animals,
pubmed-meshheading:11027578-Base Sequence,
pubmed-meshheading:11027578-Blotting, Western,
pubmed-meshheading:11027578-COS Cells,
pubmed-meshheading:11027578-Chromosomes, Human, Pair 12,
pubmed-meshheading:11027578-Cloning, Molecular,
pubmed-meshheading:11027578-Evolution, Molecular,
pubmed-meshheading:11027578-Humans,
pubmed-meshheading:11027578-Mice,
pubmed-meshheading:11027578-Molecular Sequence Data,
pubmed-meshheading:11027578-Phosphotyrosine,
pubmed-meshheading:11027578-Phylogeny,
pubmed-meshheading:11027578-Protein Structure, Tertiary,
pubmed-meshheading:11027578-Protein Tyrosine Phosphatases,
pubmed-meshheading:11027578-Radiation Hybrid Mapping,
pubmed-meshheading:11027578-Sequence Alignment,
pubmed-meshheading:11027578-Sequence Homology,
pubmed-meshheading:11027578-Terminology as Topic,
pubmed-meshheading:11027578-Transfection
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| pubmed:year |
2000
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| pubmed:articleTitle |
Isolation and characterization of murine orthologue of PTP-BK.
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| pubmed:affiliation |
Department of Molecular Genetics, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan.
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| pubmed:publicationType |
Journal Article
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