Linked Life Data

11027350

Source:http://linkedlifedata.com/resource/pubmed/id/11027350

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2000-11-9
pubmed:abstractText
The reported isolation of nanobacteria from human kidney stones raises the intriguing possibility that these microorganisms are etiological agents of pathological extraskeletal calcification [Kajander, E. O. & Ciftçioglu, N. (1998) Proc. Natl. Acad. Sci. USA 95, 8274-8279]. Nanobacteria were previously isolated from FBS after prolonged incubation in DMEM. These bacteria initiated biomineralization of the culture medium and were identified in calcified particles and biofilms by nucleic acid stains, 16S rDNA sequencing, electron microscopy, and the demonstration of a transferable biomineralization activity. We have now identified putative nanobacteria, not only from FBS, but also from human saliva and dental plaque after the incubation of 0.45-microm membrane-filtered samples in DMEM. Although biomineralization in our "cultures" was transferable to fresh DMEM, molecular examination of decalcified biofilms failed to detect nucleic acid or protein that would be expected from growth of a living entity. In addition, biomineralization was not inhibited by sodium azide. Furthermore, the 16S rDNA sequences previously ascribed to Nanobacterium sanguineum and Nanobacterium sp. were found to be indistinguishable from those of an environmental microorganism, Phyllobacterium mysinacearum, that has been previously detected as a contaminant in PCR. Thus, these data do not provide plausible support for the existence of a previously undiscovered bacterial genus. Instead, we provide evidence that biomineralization previously attributed to nanobacteria may be initiated by nonliving macromolecules and transferred on "subculture" by self-propagating microcrystalline apatite.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-10369736, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-10571799, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-10844606, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-14114874, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-4710793, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-5580215, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-6374762, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-8688069, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-8833598, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-8893307, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-9206833, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-9206834, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-9206835, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-9653101, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-9653177, http://linkedlifedata.com/resource/pubmed/commentcorrection/11027350-9687486
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11511-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
An alternative interpretation of nanobacteria-induced biomineralization.
pubmed:affiliation
Oral Infection and Immunity Branch, and Cellular Imaging Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, 20892, USA. john.cisar@nih.gov
pubmed:publicationType
Journal Article