Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-10-16
pubmed:abstractText
SHP-1 is an important negative regulator of signaling by several receptors including receptors for interleukin-2 (IL-2R) and other cytokines. SHP-1 acts by dephosphorylating the receptors and receptor-associated kinases such as IL-2R-associated Jak3 kinase. We found that SHP-1 protein was not detectable or greatly diminished in most (six of seven) T cell lines derived from various types of T cell lymphomas and all (eight of eight) cutaneous T-cell lymphoma tissues with a transformed, large-cell morphology. All T-cell lymphoma lines tested (eight of eight) expressed diminished amounts or no detectable SHP-1 mRNA. These T cell lines did not, however, carry any mutations in the SHP-1 gene-coding, splice-junction, and promoter regions. Importantly, SHP-1 DNA promoter region in the T cell lines was resistant to digestion with three different methylation-sensitive restriction enzymes. This resistance was reversed by treatment of the cells with a demethylating agent, 5-deoxyazacytidine. The treatment resulted also in the expression of SHP-1 mRNA and, less frequently, SHP-1 protein. The expression of SHP-1 protein was associated with dephosphorylation of the Jak3 kinase. These results show that lack of SHP-1 expression is frequent in malignant T cells and results from methylation of the SHP-1 gene promoter. Furthermore, they indicate that SHP-1 loss may play a role in the pathogenesis of T cell lymphomas by permitting persistence of signals generated by IL-2R and, possibly, other receptor complexes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10050851, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10066179, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10229324, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10325416, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10358776, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10463569, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-10497129, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-1373816, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-1385421, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-1606614, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-1652101, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-1732748, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-1736296, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-2758464, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7481767, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7481768, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7481769, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7528668, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7604283, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7657825, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7659163, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7665165, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7682880, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7684496, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7697543, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7777534, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7831294, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7889566, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7973658, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-7988557, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8022485, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8197173, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8324828, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8348149, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8462096, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8505313, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8506290, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8562967, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-8799169, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9048211, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9121771, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9148918, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9348315, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9391124, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9520455, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9597132, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9662389, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9852037, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9916713, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9988266, http://linkedlifedata.com/resource/pubmed/commentcorrection/11021818-9990116
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Azacitidine, http://linkedlifedata.com/resource/pubmed/chemical/DNA Modification Methylases, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/JAK3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 3, http://linkedlifedata.com/resource/pubmed/chemical/PTPN11 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/PTPN6 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/decitabine
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1137-46
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
More...