Linked Life Data

11018281

Source:http://linkedlifedata.com/resource/pubmed/id/11018281

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-12-5
pubmed:databankReference
pubmed:abstractText
In all sequenced herpesvirus genomes, a homolog of the herpes simplex virus type 1 UL15 gene has been identified. This gene encodes a protein that is involved in viral genome maturation. Although transcription of the alphaherpesvirus UL15 gene has been analyzed in detail, not much is known about the expression of its betaherpesvirus homologs. We therefore set out to characterize transcription of the rat cytomegalovirus counterpart of UL15, R89. Here we report that R89 consists of two exons separated by a 4.7-kb intron. The spliced R89 transcript, which is expressed at late times postinfection (p.i.), has the capacity to encode a protein of 670 amino acids with a calculated molecular mass of 77.1 kDa. The predicted amino acid sequence of this protein is highly similar to that of the proteins predicted to be encoded by the human cytomegalovirus UL89 and murine cytomegalovirus M89 genes (64.3 and 84.5% overall identity, respectively). The region between R89 exon 1 and exon 2 was found to contain five additional genes, r90, R91, R92, R93 and R94, the latter two of which are conserved among all herpesviruses. We show that these genes are transcribed in a highly complex fashion, resulting in numerous mono- and polycistronic mRNAs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-30
pubmed:dateRevised
2004-2-16
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Rat cytomegalovirus R89 is a highly conserved gene which expresses a spliced transcript.
pubmed:affiliation
Department of Medical Microbiology, Cardiovascular Research Institute Maastricht, University of Maastricht, PO Box 5800, 6202 AZ, Maastricht, The Netherlands.
pubmed:publicationType
Journal Article