Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-10-13
pubmed:abstractText
Development of drug resistance is the inevitable consequence of incomplete suppression of virus plasma levels in HIV-1-infected patients treated with highly active antiretroviral therapy. Resistance mutations previously characterized have been found in B subtype viruses of developed countries. Moreover, mutation profiles for non-B and more divergent B subtype viruses found in developing countries shall be analyzed together with their ex vivo phenotyping in order to establish an exact correlation between the genotyping data and the clinical management counseling for those uncommon virus subtypes. In the present study, we evaluated the mutation profile for individuals infected with B subtype and non-B subtype viruses. Viral DNA fragments corresponding to the RT gene were amplified, sequenced, and subtyped. Phenotyping analysis for reverse transcriptase nucleoside (NRTI) and nonnucleoside inhibitor susceptibility was performed using the recombinant virus assay technology. Brazilian non-B subtypes (subtype F, n = 4, and subtype A, n = 1) isolates showed essentially the same B subtype mutation profile, presenting an NRTI drug resistance with similar MIC50% and MIC90% values for all drugs analyzed regardless of their subtypes. A strong cross-resistance phenotype among AZT, 3TC, and abacavir could be seen in all isolates analyzed. A novel result was that some RT sequences not only revealed the presence of G333D/E mutations but also correlated to the presence of mutation T386I that could abrogate the M184V-surpassing effect of L210W or L210W plus G333D/E. These findings suggest that Brazilian non-B subtype HIV-1 strains use an identical RT drug resistance mutation pattern when compared to B isolates and will contribute to the validation of the genotypic and phenotypic tests in these predominant worldwide-spread viral variants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0042-6822
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-15
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11017792-Acquired Immunodeficiency Syndrome, pubmed-meshheading:11017792-Amino Acid Sequence, pubmed-meshheading:11017792-Amino Acid Substitution, pubmed-meshheading:11017792-Anti-HIV Agents, pubmed-meshheading:11017792-Brazil, pubmed-meshheading:11017792-DNA Mutational Analysis, pubmed-meshheading:11017792-Drug Resistance, Microbial, pubmed-meshheading:11017792-Drug Resistance, Multiple, pubmed-meshheading:11017792-Drug Therapy, Combination, pubmed-meshheading:11017792-Female, pubmed-meshheading:11017792-Genetic Variation, pubmed-meshheading:11017792-Genotype, pubmed-meshheading:11017792-HIV Reverse Transcriptase, pubmed-meshheading:11017792-HIV-1, pubmed-meshheading:11017792-Humans, pubmed-meshheading:11017792-Male, pubmed-meshheading:11017792-Molecular Sequence Data, pubmed-meshheading:11017792-Mutation, pubmed-meshheading:11017792-Phenotype, pubmed-meshheading:11017792-Phylogeny, pubmed-meshheading:11017792-Reverse Transcriptase Inhibitors, pubmed-meshheading:11017792-Risk Factors, pubmed-meshheading:11017792-Sequence Alignment, pubmed-meshheading:11017792-Time Factors, pubmed-meshheading:11017792-Treatment Failure
pubmed:year
2000
pubmed:articleTitle
Drug-resistant reverse transcriptase genotyping and phenotyping of B and non-B subtypes (F and A) of human immunodeficiency virus type I found in Brazilian patients failing HAART.
pubmed:affiliation
Laboratory of Molecular Virology, Genetic Department, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21944-970, Brazil.
pubmed:publicationType
Journal Article