Linked Life Data

11017775

Source:http://linkedlifedata.com/resource/pubmed/id/11017775

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-11-8
pubmed:abstractText
Expression patterns of TSH beta and FSH beta genes were investigated in normally metamorphosing and metamorphosed individuals and in goitrogen-treated (TU-SPC), metamorphosis-arrested larvae of a Japanese salamander, Hynobius retardatus, a particular population of which had been reported to show neoteny. Cloned sequences for Hynobius TSH beta and FSH beta covered 66 and 56% of full-length genes, respectively, and both showed relatively high similarity to those of other vertebrates. TSH beta-expressing cells were observed at the ventro-caudal region of the pars distalis of controls, whereas an extraordinarily large number of cells which occupied the whole area of the pituitary glands strongly expressed TSH beta in TU-SPCs. In contrast, no FSH beta-expressing cells were observed in the pituitary glands of controls, whereas several positive cells were observed in the pituitary glands of TU-SPCs. Northern blot analyses revealed a rapid increase of TSH beta expression in the pituitary gland of TU-SPCs that finally reached the level of 20- to 30-fold as much as that of controls. Surprisingly, the expression of FSH beta also increased in the pituitary gland of TU-SPCs compared with that of controls. On the other hand, triiodothyronine administration to the control juveniles reduced the expression of TSH beta compared with saline-administrated controls, suggesting that thyroid hormone (TH) exerts a repressing effect on TSH beta gene. These results suggest that TSH beta overexpression in TU-SPCs must be caused by a deprivation of TH which plays a role in the negative feedback system and that FSH beta expression also might be enhanced by mechanisms similar to those of TSH beta, probably due to certain overlapping of the endocrinological regulation of TSH and FSH in H. retardatus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0016-6480
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
276-86
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:11017775-Amino Acid Sequence, pubmed-meshheading:11017775-Animals, pubmed-meshheading:11017775-Antithyroid Agents, pubmed-meshheading:11017775-Cloning, Molecular, pubmed-meshheading:11017775-DNA, Complementary, pubmed-meshheading:11017775-Follicle Stimulating Hormone, pubmed-meshheading:11017775-Follicle Stimulating Hormone, beta Subunit, pubmed-meshheading:11017775-Gene Expression, pubmed-meshheading:11017775-Gene Expression Regulation, pubmed-meshheading:11017775-In Situ Hybridization, pubmed-meshheading:11017775-Metamorphosis, Biological, pubmed-meshheading:11017775-Molecular Sequence Data, pubmed-meshheading:11017775-Perchloric Acid, pubmed-meshheading:11017775-Pituitary Gland, pubmed-meshheading:11017775-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11017775-Sodium Compounds, pubmed-meshheading:11017775-Thiourea, pubmed-meshheading:11017775-Thyrotropin, pubmed-meshheading:11017775-Time Factors, pubmed-meshheading:11017775-Urodela
pubmed:year
2000
pubmed:articleTitle
Spatio-temporal expression of TSH beta and FSH beta genes in normally metamorphosing, metamorphosed, and metamorphosis-arrested Hynobius retardatus.
pubmed:affiliation
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
pubmed:publicationType
Journal Article