Linked Life Data

11016696

Source:http://linkedlifedata.com/resource/pubmed/id/11016696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-23
pubmed:databankReference
pubmed:abstractText
The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarum ATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparison of the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R. salmoninarum and the high G+C group of the actinobacteria, in particular, Arthrobacter species. A regulatory element within the 5' leader of the rRNA operon was identical to an element, CL2, described for mycobacteria. PCR, DNA sequence analysis, and DNA hybridisation were performed to examine variation between isolates from diverse sources which represented the four 16S-23S rRNA intergenic spacer sequevars previously described for R. salmoninarum. Two 23S-5S rRNA intergenic spacer sequevars of identical length were found. DNA hybridisation using probes complementary to 23S rDNA and 16S rDNA identified two rRNA operons which were identical or nearly identical amongst 40 isolates sourced from a variety of countries.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-6072
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-61
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11016696-Actinomycetales, pubmed-meshheading:11016696-Base Sequence, pubmed-meshheading:11016696-Conserved Sequence, pubmed-meshheading:11016696-DNA, Ribosomal, pubmed-meshheading:11016696-Gene Dosage, pubmed-meshheading:11016696-Genetic Variation, pubmed-meshheading:11016696-Gram-Positive Asporogenous Rods, Regular, pubmed-meshheading:11016696-Molecular Sequence Data, pubmed-meshheading:11016696-Operon, pubmed-meshheading:11016696-Phylogeny, pubmed-meshheading:11016696-Polymerase Chain Reaction, pubmed-meshheading:11016696-RNA, Ribosomal, pubmed-meshheading:11016696-RNA, Ribosomal, 16S, pubmed-meshheading:11016696-RNA, Ribosomal, 23S, pubmed-meshheading:11016696-RNA, Ribosomal, 5S, pubmed-meshheading:11016696-Restriction Mapping, pubmed-meshheading:11016696-Sequence Analysis, DNA
pubmed:year
2000
pubmed:articleTitle
Renibacterium salmoninarum isolates from different sources possess two highly conserved copies of the rRNA operon .
pubmed:affiliation
Department of Biological Sciences, University of Plymouth, Devon, UK. tgrayson@plymouth.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't