Linked Life Data

11016457

Source:http://linkedlifedata.com/resource/pubmed/id/11016457

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2000-10-17
pubmed:abstractText
The exact mechanism for capillary occlusion in diabetic retinopathy is still unclear, but increased leukocyte-endothelial cell adhesion has been implicated. We examined the possibility that posttranslational modification of surface O-glycans by increased activity of core 2 transferase (UDP-Glc:Galbeta1-3GalNAcalphaRbeta-N-acetylglucoaminyltr ansferase) is responsible for increased adhesion of leukocytes to vascular endothelium in diabetes. The mean activity of core 2 transferase in polymorphonuclear leukocytes isolated from type 1 and type 2 diabetic patients was higher compared with age-matched control subjects (1,638 +/- 91 [n = 42] vs. 249 +/- 35 pmol x h(-1) x mg(-1) protein [n = 24], P = 0.00013; 1,459 +/- 194 [n = 58] vs. 334 +/- 86 [n = 11], P = 0.01). As a group, diabetic patients with retinopathy had significantly higher mean activity of core 2 transferase compared with individuals with no retinopathy. There was a significant association between enzyme activity and severity of retinopathy in type 1 and type 2 diabetic patients. There was a strong correlation between activity of core 2 transferase and extent of leukocyte adhesion to cultured retinal capillary endothelial cells for diabetic patients but not for age-matched control subjects. Results from transfection experiments using human myelocytic cell line (U937) demonstrated a direct relationship between increased activity of core 2 transferase and increased binding to cultured endothelial cells. There was no relationship between activity of core 2 transferase and HbA(1c) (P = 0.8314), serum advanced glycation end product levels (P = 0.4159), age of the patient (P = 0.7896), and duration of diabetes (P = 0.3307). On the basis that branched O-glycans formed by the action of core 2 transferase participate in leukocyte adhesion, the present data suggest the involvement of this enzyme in increased leukocyte-endothelial cell adhesion and the pathogenesis of capillary occlusion in diabetic retinopathy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0012-1797
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1724-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11016457-Adult, pubmed-meshheading:11016457-Aging, pubmed-meshheading:11016457-Capillaries, pubmed-meshheading:11016457-Cell Adhesion, pubmed-meshheading:11016457-Cells, Cultured, pubmed-meshheading:11016457-Diabetes Mellitus, Type 1, pubmed-meshheading:11016457-Diabetes Mellitus, Type 2, pubmed-meshheading:11016457-Diabetic Retinopathy, pubmed-meshheading:11016457-Endothelium, Vascular, pubmed-meshheading:11016457-Female, pubmed-meshheading:11016457-Glycosylation, pubmed-meshheading:11016457-Glycosylation End Products, Advanced, pubmed-meshheading:11016457-Hemoglobin A, Glycosylated, pubmed-meshheading:11016457-Humans, pubmed-meshheading:11016457-Male, pubmed-meshheading:11016457-Middle Aged, pubmed-meshheading:11016457-N-Acetylglucosaminyltransferases, pubmed-meshheading:11016457-Neutrophils, pubmed-meshheading:11016457-Retinal Vessels, pubmed-meshheading:11016457-Transfection
pubmed:year
2000
pubmed:articleTitle
Activity of the glycosylating enzyme, core 2 GlcNAc (beta1,6) transferase, is higher in polymorphonuclear leukocytes from diabetic patients compared with age-matched control subjects: relevance to capillary occlusion in diabetic retinopathy.
pubmed:affiliation
GKT Department of Ophthalmology, The Raynes Institute, St. Thomas' Hospital, London, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't