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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-10-13
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pubmed:abstractText |
We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT(1)) A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT(1) A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1531-2267
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
31
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-3
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:11015563-Alleles,
pubmed-meshheading:11015563-DNA,
pubmed-meshheading:11015563-DNA Primers,
pubmed-meshheading:11015563-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11015563-Genotype,
pubmed-meshheading:11015563-Humans,
pubmed-meshheading:11015563-Mutagenesis,
pubmed-meshheading:11015563-Nucleic Acid Hybridization,
pubmed-meshheading:11015563-Polymerase Chain Reaction,
pubmed-meshheading:11015563-Polymorphism, Single Nucleotide,
pubmed-meshheading:11015563-Receptor, Angiotensin, Type 1,
pubmed-meshheading:11015563-Receptor, Angiotensin, Type 2,
pubmed-meshheading:11015563-Receptors, Angiotensin,
pubmed-meshheading:11015563-Reproducibility of Results
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pubmed:year |
1999
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pubmed:articleTitle |
A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism.
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pubmed:affiliation |
The Western Australian Centre for Pathology and Medical Research (PathCentre), Perth, Australia.
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pubmed:publicationType |
Journal Article
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